A composition, kit and method for detecting natural polyclonal anticancer antibodies
A composition and kit technology, applied in the field of immunology, can solve the problems of chemotherapy failure, insensitivity to initial chemotherapy drugs, etc., and achieve the effects of moderate cost, low side effects and high precision
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Examples
Embodiment 1
[0056] Example 1 uses the 4 kinds of antigenic polypeptides described in Table 1 to detect the level of NPAA in plasma
[0057] 1. Sample collection: A blood station in Guangdong Province provided 102 fresh plasma samples from normal adult blood donors. The blood donors were all individuals who had never suffered from any type of tumor as determined by clinical physical examination. All plasma samples were stored at 4 degrees (4°C) before operation, and it was verified that the storage time did not exceed 48 hours.
[0058] 2. Sample detection: The four polypeptide antigens listed in Table 1 used in this experiment were all synthesized by SEVERNBIOTECH Co., Ltd., UK, with a purity of 95%. The specific operation steps are as follows:
[0059] (1) Before operation, each antigen was dissolved in 67% acetic acid into a 5.0 mg / ml stock solution, then mixed in equal volumes and stored in a -20°C (within ±2°C) refrigerator.
[0060] (2) At the beginning of the operation, at first t...
Embodiment 2
[0074] Example 2 Effect of NPAA-rich plasma on tumor cells
[0075] The A549 lung cancer cell line is derived from pneumocytic basal adenocarcinoma, which is a passage cell line; the A549 lung cancer cell line can be stably subcultured, and has been widely used to screen anti-tumor drugs and verify tumor treatment methods, and is an ideal pre-tumor clinical research materials. In this example, the A549 lung cancer cell line was cultured with NPAA-rich and NPAA-free plasma, and the inhibitory effect of NPAA on the proliferation of the cell line was observed.
[0076] 1. Plasma preparation for cell culture: 3 bags of NPAA-enriched and NPAA-free plasma obtained in Example 1 were randomly selected. Mix separately under aseptic conditions, and store in a refrigerator at minus 20 degrees (-20°C) after sub-packaging.
[0077] 2. The cultivation of tumor cell lines: the A549 lung cancer cell lines (obtained from the cell bank of Guangdong Medical College) were divided into two group...
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Description
Claims
Application Information
- IPC
- C07K14/47
- CPC
- G01N21/31; G01N33/57473
- Inventors
- 尉军; 孟庆勇