Engineered leather and methods of manufacture thereof

一种皮革、工程的技术，应用在工程的皮革和其制造领域，能够解决缺乏天然皮革质量、耐久性和声望、没有开发出科学上合理天然皮革等问题

Inactive Publication Date: 2015-05-06
MODERN MEADOW INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although synthetic leather was developed to address these issues, it lacks the quality, durability and prestige of natural leather
To date, no scientifically sound and industrially viable process has been developed for the preparation of natural leather

Method used

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  • Engineered leather and methods of manufacture thereof
  • Engineered leather and methods of manufacture thereof
  • Engineered leather and methods of manufacture thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0173] Embodiment 1 - preparation of support substrate

[0174] To prepare a 2% agarose solution, 2 g of ultrapure low melting point (LMP) agarose was dissolved in 100 mL of ultrapure water / buffer (1:1, v / v). The buffer is optionally PBS (Dulbecco's Phosphate Buffered Saline lx) or HBSS (Hanks Balanced Salt Solution lx). The agarose solution was placed in a beaker with warm water (over 80°C) and placed on a hot plate until the agarose was completely dissolved. As long as the temperature exceeds 36°C, the agarose solution remains liquid. Below 36°C, a phase transition occurs, viscosity increases and eventually the agarose forms a gel.

[0175] To prepare the agarose support substrate, 10 mL of liquid 2% agarose (temperature >40° C.) was placed in a 10 cm diameter petri dish and spread evenly to form a consistent layer. Agarose was allowed to form a gel in a refrigerator at 4 °C.

Embodiment 2

[0176] Example 2 - Culture of bovine keratinocytes, fibroblasts and epithelial cells

[0177] Fresh isolated bovine keratinocytes, fibroblasts and epithelial cells were grown in low glucose DMEM with 10% calf serum (Hyclone Laboratories, UT), 10% porcine serum (Invitrogen) , L-Vitamin C, Copper Sulfate, HEPES, L-Proline, L-Alanine, L-Glycine, and Penicillin G (all aforementioned supplements from Sigma, St.Louis, MO purchase). Cell lines were cultured on Petri dishes (Techno Plastic Products, St. Louis, MO) coated with less than 0.5% gelatin (porcine skin gelatin; Sigma) and maintained at 37°C in the presence of 5% CO. 2 in the humid air. Keratinocytes were cultured again to passage 7 before being used to form multicellular bodies.

Embodiment 3

[0178] Example 3 - Preparation of multicellular spheroids and cylinders

[0179] Cell cultures were washed twice with phosphate-buffered saline (PBS, Invitrogen), treated with 0.1% insulin (Invitrogen) for 10 minutes, and then centrifuged at 1500 RPM for 5 minutes. Cells were resuspended in 4 mL of cell-type specific media and then incubated at 37 °C with 5% CO 2 Incubate in a 10 Ml tissue culture flask of a 100 ml tissue culture flask and continue for 1 hour on a rotary shaker (New Brunswick Scientific, Edison, NJ) for adhesion recovery, and centrifuge at 3500 RPM. The resulting pellet was transferred to a 300 μm (Sutter Instrument, CA) or 500 μm (Drummond Scientific Company, Broomall, PA) diameter capillary micropipette at 37°C with 5% CO 2 Incubate for 15 minutes. For spherical multicellular bodies, extruded cylinders were cut into equal pieces, which were collected overnight on a rotary shaker. Depending on the diameter of the micropipette, this step provides conventi...

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PUM

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Abstract

Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and / or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.

Description

[0001] Cross References to Related Applications [0002] This application is claiming priority to US Provisional Patent Application No. 61 / 616,888, filed March 28, 2012, entitled "ENGINEERED LEATHER AND METHODS OF MANUFACTURE THEREOF," which is hereby incorporated by reference in its entirety. [0003] incorporated by reference [0004] All publications and patent applications mentioned in this specification are herein incorporated by reference as if each individual publication or patent application was specifically or individually indicated to be incorporated by reference. Background technique [0005] Leather is used in a large variety of applications including upholstery, apparel, footwear, luggage, handbags and accessories, and automotive applications. Currently, animal fur is used as a raw material for natural leather. However, fur from livestock creates environmental concerns because raising livestock requires large amounts of feed, pasture, water and fossil fuels. Li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C14C3/00C14B1/30C14B1/56
CPCB32B9/025C12N5/0698C14B7/00C08H1/06C08L89/06C12N5/0062C12N2533/76C12N2533/90C12P21/00
Inventor G·福加斯F·马尔加K·雅各布
Owner MODERN MEADOW INC
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