Ganoderma lucidum total triterpene purified product with high anthropogenic tumor cell activity and purification method of ganoderma lucidum total triterpene purified product

Abstract

The invention discloses a ganoderma lucidum total triterpene purified product with high anthropogenic tumor cell activity and a purification method of the ganoderma lucidum total triterpene purified product. The method comprises the following steps: purifying a ganoderma lucidum extract liquid with macroporous resin and MCI GEL resin, destaining, concentrating a destaining solution and then carrying out spray drying, so as to obtain the ganoderma lucidum total triterpene purified product with obvious anthropogenic tumor cell activity. The ganoderma lucidum total triterpene purified product has the advantages that the preparation method is simple and fast, free of a toxic solvent and free of harm to a human body and environment; the purified product is low in production cost, stable in quality, and suitable for industrial production; and the purified ganoderma lucidum total triterpene has obvious anthropogenic tumor cell activity, and can be further developed into anticancer medicines.

Description

technical field [0001] The invention relates to a purified ganoderma total triterpenes with high anti-human tumor cell activity and a purification method thereof, in particular to a purification method of ganoderma total triterpenes by using macroporous resin, MCI GEL resin and polyamide. Background technique [0002] Ganoderma lucidum is the dry fruiting body of the polyporaceae fungus Chizhi or Zizhi. Modern research shows that the main chemical components of Ganoderma lucidum include sugars, triterpenoids, sterols, nucleosides, amino acids and alkaloids, etc. Pharmacological activity, so it is developed as medicine, food and health care product. [0003] With the development of modern society, the number of cancer patients is increasing year by year, and it has become a disease that people urgently need to overcome. Triterpenoids are the main anti-tumor substances in Ganoderma lucidum, which can inhibit the division and proliferation of tumor cells, inhibit the invasio...

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Problems solved by technology

[0004] At present, in the purification technology of Ganoderma lucidum triterpenes, the prior art mostly adopts organic solvent extraction, such as patent CN1546519A, which uses reagent chloroform and its saturated sodium bicarbonate solution for extraction, but this method not only uses toxic reagents, but also cannot maintain Ganoderma lucidum triterpenes. Integrity of active ingredients; Publication No. CN102772448A, although this method has adopted macroporous resin to purify Ganoderma triterpenoids to keep the integrity of active ingredients of Ganoderma lucidum triterpenes, but this method uses a large amount of acid and alkali, which not only damages the resin more Large, and the use of acid and alkali can also lead to changes in substances, which is not suitable for long-term industrial production; the pigment color of Ganoderma lucidum extract is darker, which has a greater impact on the content of components. Currently, activated carbon is often used for decolorization, but in the decolorization process of this method , activated carbon will adsorb a large amount of ganoderma triterpenes, seriously affecting the yield of ganoderma triterpenoids

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