Colletotrichum lini strain LJYH20130405-1 for steride fermentation as well as application technology thereof
A technology of Nechosporium flaxenum and steroids, which is applied in the field of high-yielding steroid fermentation of Nechosporium linenensis strains and the fermentation of steroidal compounds, can solve the problems of high extraction and purification yields, and achieve stable biological properties , Reduce production cost, increase output effect
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[0060] Example: For the new strain of Neptonia flaxensis proposed by the present invention, 5 examples were verified for the entire process of its strain culture medium, culture conditions and shaker verification (converting DHEA into 7α, 15α-dihydroxyandrostenolone by fermentation) . The results showed that the solid-phase conversion rate of DHEA and the 7α, 15α-dihydroxyandrostene of the strain LJYH20130405-1 were changed under the conditions of corresponding changes in the medium and culture conditions (shaking table speed and culture temperature). The yields of alcohols and ketones are respectively stable between 83~91% and 78~82%, illustrating that the novel strain of Nephrospora flaxensis proposed by the present invention has excellent characters (see Table 1); The strain also has a high conversion rate and yield, the conversion rate of DHEA is 83-86%, and the yield of 7α, 15α-dihydroxyandrostenolone (excellent product) is 78-85% (see Table 2).
[0061] Table 1 Compar...
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