Colletotrichum lini strain LJYH20130405-1 for steride fermentation as well as application technology thereof

A technology of Nechosporium flaxenum and steroids, which is applied in the field of high-yielding steroid fermentation of Nechosporium linenensis strains and the fermentation of steroidal compounds, can solve the problems of high extraction and purification yields, and achieve stable biological properties , Reduce production cost, increase output effect

Inactive Publication Date: 2015-06-03
丽江映华生物药业有限公司
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Problems solved by technology

The experimental results show that the conversion rates of direct conversion and indirect conversion are both 60

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  • Colletotrichum lini strain LJYH20130405-1 for steride fermentation as well as application technology thereof
  • Colletotrichum lini strain LJYH20130405-1 for steride fermentation as well as application technology thereof

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[0060] Example: For the new strain of Neptonia flaxensis proposed by the present invention, 5 examples were verified for the entire process of its strain culture medium, culture conditions and shaker verification (converting DHEA into 7α, 15α-dihydroxyandrostenolone by fermentation) . The results showed that the solid-phase conversion rate of DHEA and the 7α, 15α-dihydroxyandrostene of the strain LJYH20130405-1 were changed under the conditions of corresponding changes in the medium and culture conditions (shaking table speed and culture temperature). The yields of alcohols and ketones are respectively stable between 83~91% and 78~82%, illustrating that the novel strain of Nephrospora flaxensis proposed by the present invention has excellent characters (see Table 1); The strain also has a high conversion rate and yield, the conversion rate of DHEA is 83-86%, and the yield of 7α, 15α-dihydroxyandrostenolone (excellent product) is 78-85% (see Table 2).

[0061] Table 1 Compar...

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Abstract

The invention discloses a high-yielding colletotrichum lini strain LJYH20130405-1 for steride fermentation as well as an application technology of the strain. A taxonomic status of the strain LJYH20130405-1 is that the sexual generation belongs to the fungi, ascomycota, sordariomycetes, sordariomycetidae, small nectriaceae and glomerella; the strain is preserved in (China General Microbiological Culture Collection Center on June 8th, 2013, with the preservation number of CGMCC 7715. According to the colletotrichum lini strain, 7alpha, 15alpha-dyhydroxyl androstenolone can be produced by using dehydroepiandrosterone (DHEA) as a substrate in a conversion process of producing plant hormones by using steroids as raw materials. The produced 7alpha, 15alpha-dyhydroxyl androstenolone has the characteristics of high conversion rate, high yield, comparatively stable biological properties and the like. According to the colletotrichum lini strain for steride fermentation, the technical support can be provided for improving the production yield of plant steroid hormone using dioscin as a raw material and reducing the cost.

Description

technical field [0001] The invention belongs to the technical field of industrial fungi, and specifically designs a high-yielding steroid-fermenting Mycelia spinosa strain and an application technology for steroidal compound fermentation. Background technique [0002] steroid biofermentation [0003] Drospirenone is an aldosterone antagonist that has anti-mineralcorticosterone activity. It is used in combination with ethinyl estradiol to form Yasmin, a new and highly effective oral contraceptive. Yasmin (drospirenone plus ethinyl estradiol) is an oral contraceptive successfully developed by the French company Schering and has been marketed in the United States and Europe. 7α, 15α-dihydroxyandrostenolone (7α, 15α-dihydroxyandrostenolone) is an important intermediate in the synthesis of drospirenone. Its production methods include traditional chemical synthesis, semi-synthesis, and biotransformation. Traditional chemical synthesis requires nearly ten steps of reaction, an...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P33/06C12R1/645
CPCC12P33/06C12N1/145C12R2001/645
Inventor 王钱钢周德群杨晓亮赵月华
Owner 丽江映华生物药业有限公司
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