A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan Powder
A technology of dimethyl ellagic acid and trimethyl ellagic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable accurate measurement and large deviation of measurement results, so as to control quality and improve accuracy , to avoid the effect of interference
Image
Examples
Embodiment 1-10
[0043] A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan powder medicinal materials, wherein the Chishan powder medicinal materials are harvested in autumn 10 batches, including the following steps:
[0044] (1) Preparation of sample solution: Take Chishan powder, crush it, pass through a 60-mesh sieve, weigh 1.0g, weigh it accurately, put it in a container, add 100ml of ethanol for reflux extraction for 1 hour, recover the solvent in the extract and concentrate to In extract form, add 20ml of water, ultrasonically treat for 20min, shake and extract with ethyl acetate 5 times, the dosage of ethyl acetate is 20ml each time, evaporate the ethyl acetate extract to dryness, add methanol to dissolve the residue and dilute to 10ml bottle, shake well, and filter through a 0.45 μm microporous membrane to obtain a sample solution;
[0045] (2) Determination of sample content:
[0046] Chromatographic conditions:
[0047] C18 c...
Embodiment 11-12
[0057] Quantitative detection method of 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan powder medicinal materials, in which Chishan powder medicinal materials are two harvested in summer batch, including the following steps:
[0058] (1) Preparation of sample solution: Take Chishan powder, crush it, pass through a 60-mesh sieve, weigh 1.0g, weigh it accurately, put it in a container, add 100ml of ethanol for reflux extraction for 1 hour, recover the solvent in the extract and concentrate to In extract form, add 20ml of water, ultrasonically treat for 20min, shake and extract with ethyl acetate 5 times, the dosage of ethyl acetate is 20ml each time, evaporate the ethyl acetate extract to dryness, add methanol to dissolve the residue and dilute to 10ml bottle, shake well, and filter through a 0.45 μm microporous membrane to obtain a sample solution;
[0059] (2) Determination of sample content:
[0060] Chromatographic conditions:
[0061] C18 chromato...
Embodiment 13-14
[0071] Quantitative detection method of 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan powder medicinal materials, in which Chishan powder medicinal materials are two harvested in spring batch, including the following steps:
[0072] (1) Preparation of sample solution: Take Chishan powder, crush it, pass through a 60-mesh sieve, weigh 1.0g, weigh it accurately, put it in a container, add 100ml of ethanol for reflux extraction for 1 hour, recover the solvent in the extract and concentrate to In extract form, add 20ml of water, ultrasonically treat for 20min, shake and extract with ethyl acetate 5 times, the dosage of ethyl acetate is 20ml each time, evaporate the ethyl acetate extract to dryness, add methanol to dissolve the residue and dilute to 10ml bottle, shake well, and filter through a 0.45 μm microporous membrane to obtain a sample solution;
[0073] (2) Determination of sample content:
[0074] Chromatographic conditions:
[0075] C18 chromato...
PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- IPC
- G01N30/02
- Inventors
- 李莉; 黄家宇