A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan Powder

A technology of dimethyl ellagic acid and trimethyl ellagic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable accurate measurement and large deviation of measurement results, so as to control quality and improve accuracy , to avoid the effect of interference

Active Publication Date: 2016-08-17
GUIYANG MEDICAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0005] Chishan powder is now included in the 2003 edition of "Quality Standards for Traditional Chinese and Ethnic Medicinal Materials in Guizhou Province". The current public literature only involves the properties and identification of Chishan powder. For the content determination of its active ingredients, only spectrophotometry is currently available. According to the literature report on the determination of the total flavonoids content in Chishan Powder, the method is based on the absorbance measured after color development, and the content of color-developing substances is generally calculated as the content of total flavonoids. The specificity and accuracy are low, and the measurement results have large deviations. conducive to accurate measurement

Method used

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  • A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan Powder
  • A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan Powder
  • A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan Powder

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Effect test

Embodiment 1-10

[0043] A quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan powder medicinal materials, wherein the Chishan powder medicinal materials are harvested in autumn 10 batches, including the following steps:

[0044] (1) Preparation of sample solution: Take Chishan powder, crush it, pass through a 60-mesh sieve, weigh 1.0g, weigh it accurately, put it in a container, add 100ml of ethanol for reflux extraction for 1 hour, recover the solvent in the extract and concentrate to In extract form, add 20ml of water, ultrasonically treat for 20min, shake and extract with ethyl acetate 5 times, the dosage of ethyl acetate is 20ml each time, evaporate the ethyl acetate extract to dryness, add methanol to dissolve the residue and dilute to 10ml bottle, shake well, and filter through a 0.45 μm microporous membrane to obtain a sample solution;

[0045] (2) Determination of sample content:

[0046] Chromatographic conditions:

[0047] C18 c...

Embodiment 11-12

[0057] Quantitative detection method of 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan powder medicinal materials, in which Chishan powder medicinal materials are two harvested in summer batch, including the following steps:

[0058] (1) Preparation of sample solution: Take Chishan powder, crush it, pass through a 60-mesh sieve, weigh 1.0g, weigh it accurately, put it in a container, add 100ml of ethanol for reflux extraction for 1 hour, recover the solvent in the extract and concentrate to In extract form, add 20ml of water, ultrasonically treat for 20min, shake and extract with ethyl acetate 5 times, the dosage of ethyl acetate is 20ml each time, evaporate the ethyl acetate extract to dryness, add methanol to dissolve the residue and dilute to 10ml bottle, shake well, and filter through a 0.45 μm microporous membrane to obtain a sample solution;

[0059] (2) Determination of sample content:

[0060] Chromatographic conditions:

[0061] C18 chromato...

Embodiment 13-14

[0071] Quantitative detection method of 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan powder medicinal materials, in which Chishan powder medicinal materials are two harvested in spring batch, including the following steps:

[0072] (1) Preparation of sample solution: Take Chishan powder, crush it, pass through a 60-mesh sieve, weigh 1.0g, weigh it accurately, put it in a container, add 100ml of ethanol for reflux extraction for 1 hour, recover the solvent in the extract and concentrate to In extract form, add 20ml of water, ultrasonically treat for 20min, shake and extract with ethyl acetate 5 times, the dosage of ethyl acetate is 20ml each time, evaporate the ethyl acetate extract to dryness, add methanol to dissolve the residue and dilute to 10ml bottle, shake well, and filter through a 0.45 μm microporous membrane to obtain a sample solution;

[0073] (2) Determination of sample content:

[0074] Chromatographic conditions:

[0075] C18 chromato...

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Abstract

The invention discloses a quantitative determination method for 3,3'-dimethyl ellagic acid and 3,3',4'-trimethyl ellagic acid in polygonum runcinatum. The quantitative determination method comprises the following steps: measuring the peak area by employing a high performance liquid chromatography under a specific chromatographic condition, and then calculating the content of the 3,3'-dimethyl ellagic acid and the 3,3',4'-trimethyl ellagic acid in the polygonum runcinatum through a linear equation. The quantitative determination method has the characteristics that the medicinal material quality of the polygonum runcinatum can be effectively controlled by quantitative determination of the 3,3'-dimethyl ellagic acid and the 3,3',4'-trimethyl ellagic acid in the polygonum runcinatum; and the method is easy to operate, stable in result and high in accuracy.

Description

technical field [0001] The invention belongs to the technical field of quantitative detection of drugs, and in particular relates to a quantitative detection method for 3,3'-dimethylellagic acid and 3,3',4'-trimethylellagic acid in Chishan Powder. Background technique [0002] 3,3'-Dimethylellagic acid and 3,3',4'-trimethylellagic acid belong to the derivatives of ellagic acid, which widely exist in the plant kingdom and have strong anti-lipid peroxidation and The ability to scavenge free radicals can inhibit a variety of bacteria and viruses, and can inhibit the canceration of liver, kidney and other parts due to chemical induction. It also has blood coagulation function. [0003] Chishan powder is derived from the dried rhizome of Polygonum runcinatum Buch.-Ham.var.sinene Hemsl., a plant of the genus Polygonum genus Polygonum runcinatum Buch.-Ham.var.sinene Hemsl. Excavated in early summer or autumn, cold in nature, bitter, astringent, slightly sour. It has the effects o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 李莉黄家宇刘青许超飞
Owner GUIYANG MEDICAL UNIVERSITY
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