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Image acquisition device and method for measuring slide inclination
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An image acquisition device and a technology for slides, which are applied to measurement devices, optical devices, microscopes, etc., can solve the problems of blurred image corners, blurry, and difficult object autofocusing.
Inactive Publication Date: 2017-04-26
SONY CORP
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However, on the other hand, when using a large image sensor, one corner in the image can be blurred and it can be difficult to autofocus on the subject, which is problematic
Blurring of a corner in an image refers to a phenomenon that, if the area of the image captured at one time is large and if the sample within the slide is meandered by about several micrometers up and down, the part of the sample on the corner of the image captured area Within the depth of focus (e.g., about 0.2 microns to 0.6 microns) such that the corners of the image are blurred
[0007] Also, a warped slide will create the same problem as a serpentine sample
Method used
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example 1
[0184] Next, as a development example of a method using an altimeter, a method of approximating the inclination of several curved slides 16 based on a plurality of planes and measuring the inclination will be described.
[0185] Figure 17 is a diagram showing a method of approximating the inclinations of several curved slides 16 based on a plurality of planes and measuring the inclinations.
[0186] (1) First, the controller measures the height of the measurement point 1 and the height of the measurement point 2 . The controller then linearly interpolates between the two measured heights.
[0187] (2) Next, the controller measures the height of the measurement point 3 .
[0188] Here, if the difference between the measured height and the height obtained by linear interpolation is within a predetermined reference value, the controller determines that the curvature is not serious, and measures and corrects the inclination of the slide 16 by the method described above using th...
example 2
[0195] Next, as another development example of the method using the altimeter, a method of approximating the inclination of several curved slides 16 based on a quadratic equation and measuring the inclination will be described.
[0196] Figure 18 is a diagram showing a method of approximating the inclinations of several curved slides 16 based on a quadratic equation and measuring the inclinations.
[0197] (1) First, the controller measures the heights of the measurement point 1, the measurement point 2, and the measurement point 3.
[0198] (2) Next, the controller derives an approximate quadratic equation based on the obtained three altitudes by using the method described above. The inclination in the X-axis direction is corrected using a quadratic equation.
[0199] (3) Next, the controller measures the height of the measurement point 4, linearly interpolates between the height of the measurement point 4 and the height of the measurement point 3, and obtains the inclinat...
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Abstract
[Object] To provide an image obtaining apparatus and a method of measuring an inclination of a slide, capable of measuring an inclination of a slide glass in a short time. [Solving Means] An image obtaining apparatus includes: a stage on which a slide is to be mounted, the slide being obtained by applying a sample to a slide glass and covering the sample with a cover glass; a stage moving unit configured to move the stage; a detection unit configured to detect a position of the sample on the slide; an optical angle measuring apparatus configured to irradiate the slide glass mounted on the stage with laser light from the side opposite to the side on which the sample is mounted, the optical angle measuring apparatus being capable of detecting an inclination of the slide glass; and a controller configured to control the stage moving unit such that the position of the sample on the slide is irradiated with the laser light, and to cause the optical angle measuring apparatus to detect the inclination.
Description
technical field [0001] This technology relates to an image acquisition device (scanner) and a method for measuring the inclination of a slide in the fields of medical treatment, pathology, biology, etc., which are used to correct the inclination of the slide when taking images of samples placed on the slide and Generate pathology images. Background technique [0002] In the fields of medical treatment, pathology, etc., there is a proposed pathological diagnosis system in which images of cells, tissues, organs, etc. tissue, etc. or make a diagnosis of a patient. In this system, a virtual slide scanner (hereinafter, simply referred to as "scanner") device is configured to take an image of a sample mounted on a glass slide and generate a digital image (virtual slide). [0003] In the field of pathological diagnosis, not only the quality of digital images needs to be improved, but also the speed at which images can be captured needs to be increased. The reason is that since i...
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