Extraction separation and identification method of chemical components of stem of Cymbaria dahurica

A technology of chemical composition and extraction liquid, which is applied in the field of extraction, separation and identification of chemical composition of the stems of Dauri cinnabarba, and can solve the problems of unreported chemical composition and biological activity.

Inactive Publication Date: 2015-07-08
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since then, the chemical composition and biologi

Method used

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  • Extraction separation and identification method of chemical components of stem of Cymbaria dahurica
  • Extraction separation and identification method of chemical components of stem of Cymbaria dahurica
  • Extraction separation and identification method of chemical components of stem of Cymbaria dahurica

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Experimental program
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Effect test

Embodiment 1

[0023] The extraction and separation of the chemical components of the stem part of Dauri simba is carried out according to the following steps:

[0024] 1. Take 700g of the stem part of Dauri simba that has been dried in the shade, crush it, heat and reflux extraction with 5 times the amount of 95% ethanol, extract 3-4 times, each time for about 3-4 hours, combine the extracts, and concentrate under reduced pressure Afterwards, a dark green extract is obtained. Disperse the extract in 95% ethanol solution as little as possible, then mix the sample with diatomaceous earth 1:1, evaporate the solvent, and extract with petroleum ether and chloroform successively for 3 times, 3 hours each time. The chloroform extract was concentrated under reduced pressure to obtain chloroform extract, which was dried.

[0025] 2. The chloroform extract obtained above is fully dissolved with as little as possible 95% ethanol, mixed with 20 g of silica gel (100-200 mesh), evaporated to dryness, an...

Embodiment 2

[0027] A method for identifying the chemical components of the stem of Dauri simba, which is to dissolve the yellow oil I and yellow oil II obtained by the extraction and separation method in Example 1 with n-hexane, and use a gas chromatography-mass spectrometer with a model number of 7890A / 5975C I and II were separated and identified with an instrument. The chromatographic conditions are as follows, chromatographic column: HP-50MS 30m*25um*0.25um, temperature control: keep at 40°C for 1min, raise to 290°C at a speed of 15°C / min, keep for 10min, inlet temperature: 290°C, sample Sample method: Agilent autosampler, splitless, injection 1μL, carrier gas: He; mass spectrometry conditions: ionization method: EI, electron, acceleration voltage: 70V, ion source temperature: 230°C, scan method: full scan 50amu ~550amu, inlet temperature: 260°C, auxiliary channel temperature: 280°C. The collected mass spectrum was searched and analyzed by NIST standard mass spectrum sequence. Yellow ...

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Abstract

The invention relates to an extraction separation and identification method of chemical components of stem of Cymbaria dahurica and belongs to the technical field of extraction separation and identification of chemical components of Chinese herbal medicines. The method comprises the following steps of taking a certain amount of dried and crushed stem of Cymbaria dahurica, carrying out crude extraction by ethanol with a concentration of 95%, carrying out Soxhlet extraction by petroleum ether and chloroform, carrying out silica gel column chromatography separation on the obtained chloroform to obtain a yellow oily material I and a yellow oily material II, and carrying out analysis on the oily materials by a (7890A/5975C) gas chromatograph-mass spectrometer, wherein 17 chemical components of the yellow oily material I and 21 chemical components of the yellow oily material II are identified. Through the method, effective components such as vitamin E, benzothiazole, limonene, beta-ionone, farnesol, dihydroactinidiolide and phytone in the mongolian medicine Cymbaria dahurica are identified at first. The method lays the foundation of development and utilization of ethnodrugs.

Description

technical field [0001] The invention belongs to the technical field of extraction, separation and identification of chemical components of Chinese herbal medicines, and in particular relates to the extraction, separation and identification of chemical components of the stem of Dawu Lishenba. Background technique [0002] Cymbaria dahurica L. is a plant of the genus Cymbaria in the family Scrophulariaceae. Mongolian medicine name: Hanqinse Rigao, aliases: big yellow flower, white Artemisia tea, Harmony is a forehead cloth Si, a perennial herb, belongs to xerophyte, born in typical grasslands, desert grasslands and mountain grasslands, widely distributed in the cities of Inner Mongolia, the three northeastern provinces, Hebei and other places; also in Siberia and Mongolia. Its roots, stems, leaves, and flowers can be used as medicine, which can dispel rheumatism, diuresis, and stop bleeding. Dawuli Xinba is widely distributed, rich in resources, and has a large content. [0...

Claims

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Application Information

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IPC IPC(8): C07D311/72C07D277/62C07C13/21C07C7/00C07C49/21C07C45/78C07C33/02C07C29/76C07D307/83C07C49/04
CPCC07D311/72C07C7/00C07C9/15C07C11/02C07C11/12C07C13/20C07C13/21C07C29/76C07C33/02C07C45/78C07C49/04C07C49/203C07C49/21C07C49/563C07C51/64C07C57/72C07C67/48C07C69/78C07C69/96C07C2601/16C07C2603/18C07D207/40C07D217/02C07D277/62C07D307/83
Inventor 王鸿宇田福利扈颖慧王璐盛万里
Owner INNER MONGOLIA UNIVERSITY
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