Silver nanoparticle, SERS active substrate, preparation method and application of SERS active substrate

Abstract

The invention provides a silver nanoparticle, an SERS (Surface Enhanced Raman Scattering) active substrate, a preparation method and application of the SERS active substrate. The preparation method comprises the following steps: adding a sodium citrate aqueous solution with a mass fraction of 2% into a boiling silver nitrate solution with a mass fraction of 0.02% drop by drop under the stirring state, 20 minutes later, cooling the solution to be at the room temperature, so as to obtain the silver nanoparticle solution, concentrating the silver nanoparticles, then mixing the nanoparticles with a pretreated functionalized adsorption carrier, leaving to stand for 6 hours at the room temperature, removing moisture from the adsorption carrier, obtaining the functionalized adsorption carrier of the silver nanoparticle, performing vacuum drying at the temperature of 30-40 DEG C, and obtaining the SERS active substrate. The SERS active substrate can be used for on-site quick test of the pesticide residual volume of fruits and vegetables in a swab or test paper manner. The SERS active substrate disclosed by the invention is simple in preparation process, low in price, good in enhancing effect, high in repeatability, portable and disposable, can avoid cross contamination of the different fruit and vegetable samples in the detection process, and benefits to rapid detection and screening of the pesticide residues on the surfaces of the large-scale fruit and vegetable samples outside an laboratory.

Description

technical field [0001] The invention belongs to the technical field of food safety monitoring, and in particular relates to a silver nanoparticle, a SERS active substrate, a preparation method and an application thereof. Background technique [0002] Pesticides are used to prevent, suppress or eliminate harmful organisms and diseases that harm crops during the growth of vegetables, fruits and other crops, and promote the vigorous development of the vegetable and fruit industry. However, pesticide residues will cause harm to the health of the eaters, and in severe cases, it will cause physical discomfort, vomiting, diarrhea and even death. Relevant experiments have shown that it is difficult to eradicate excessive pesticides remaining on the surface of fruits and vegetables by washing with water or washing with detergent. Therefore, it is necessary to strengthen the detection of pesticide residues on the surface of fruits and vegetables to ensure the safety of consumers of fr...

AI Technical Summary

Problems solved by technology

However, most of these methods rely on professional equipment, cumbersome preparation process, many interference factors, and high preparation costs. These unfavorable factors limit the application of SERS technology in actual detection and analysis.

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