Enrichment culture medium

A technology of enrichment culture medium and bovine liver powder, which is applied in the field of medical inspection, can solve the problems of increased mortality rate of nocardiosis, delay in accurate diagnosis of nocardiosis, and huge impact of nocardiosis diagnosis, and achieve isolation rate High and reliable detection effect

Inactive Publication Date: 2015-08-19
钟学文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing culture methods have the following problems: ①Because Nocardia is a fastidious bacterium, that is, bacteria that have strict requirements on the growth environment and nutrition, they cannot or are difficult to grow in ordinary environments, so it takes 7 days to complete a reliable diagnosis , often delaying the accurate clinical diagnosis of nocardiosis and the timely adoption of effective treatment measures, resulting in an increase in the fatality rate of nocardiosis; Inhibition, which leads to a low positive rate of Nocardiosis isolation and culture has a huge impact on the diagnosis of Nocardiosis

Method used

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  • Enrichment culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0010] Example 1, the formula is: beef liver powder 10.0g; malt extract powder 2.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; ferric ammonium citrate 0.1g; calcium lactate 0.05g; Cycloserine 0.05g; Trichosanone 0.001g; Vitamin K 1 0.1g; Sterile defibrinated sheep blood 100ml; Distilled water added to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferric ammonium citrate; calcium lactate; , add sterilized D-cycloserine; sterilized vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.

Embodiment 2

[0011] Example 2, the formula is: beef liver powder 10.0g; malt extract powder 5.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; ferric ammonium citrate 0.3g; calcium lactate 0.03g; Cycloserine 0.03g; Trichosanone 0.003g; Vitamin K 1 0.01g; complexin phosphopeptide 0.1g; sterile defibrinated sheep blood 80ml;; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferric ammonium citrate; calcium lactate; ℃, add sterilized D-cycloserine; sterilized vitamin K 1 ; Sterilized Collin Phosphopeptide; Sterile defibrinated sheep blood mixed evenly, sterilized distilled water to 1000ml before packing.

Embodiment 3

[0012] Example 3, the formula is: beef liver powder 5.0g; malt extract powder 3.0g; yeast extract powder 10.0g; sodium chloride 5.0g; agar 20.0g; ferric ammonium citrate 0.2g; calcium lactate 0.03g; Cycloserine 0.01g; Trichosanone 0.001g; Vitamin K 1 0.03g; 0.3g of complexin phosphopeptide; 90ml of sterile defibrinated sheep blood; 250ml of rehmannia root water extract with a concentration of 200g / L; Preparation method Preparation method: (1) Take 50g of rehmannia glutinosa and add water to boil for 45 minutes, filter, take the filtrate, and according to the volume of the filtrate, concentrate or add water to adjust the volume to a concentration of 200g / L to obtain 250ml of rehmannia glutinosa water extract; (2) weigh Take bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferric ammonium citrate; calcium lactate; Sterilize at ℃ for 15 minutes, cool to 45℃, add sterilized D-cycloserine; sterilized colulin phosphopeptide; sterilized vitamin...

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Abstract

The invention discloses an enrichment culture medium and belongs to the field of detection. The enrichment culture medium is characterized in that a formula contains beef liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferric ammonium citrate, calcium lactate, D-seromycin, 2-ethoxymethylene-3,5-dihydroxy-y-pyrone, vitamin K1, sterile defiberized sheep blood and distilled water. Compared with the prior art, the enrichment culture medium plays roles in transporting and enriching.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a culture medium for enriching bacteria. Background technique [0002] Norcardosis is a zoonotic chronic disease caused by bacteria of the genus Nocardia, characterized by tissue suppuration, necrosis or abscess formation. The disease is widely distributed all over the world, and pulmonary nocardiosis is the most common. Nocardia can cause primary and purulent lung infection in people through the respiratory tract, and symptoms of pulmonary tuberculosis can occur. Lung lesions can metastasize to the subcutaneous tissue, forming abscesses, ulcers, and multiple fistulas, and can also spread to other organs. The clinical symptoms are similar to pulmonary tuberculosis, pulmonary aspergillosis, and pulmonary Weiger's granuloma, so it is easy to misdiagnose. [0003] The clinical diagnosis of the disease mostly relies on bacterial culture. The specific method is: after 3 days of cultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 钟学文
Owner 钟学文
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