Enrichment culture medium
A technology of enrichment culture medium and bovine liver powder, which is applied in the field of medical inspection, can solve the problems of increased mortality rate of nocardiosis, delay in accurate diagnosis of nocardiosis, and huge impact of nocardiosis diagnosis, and achieve isolation rate High and reliable detection effect
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Embodiment 1
[0010] Example 1, the formula is: beef liver powder 10.0g; malt extract powder 2.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; ferric ammonium citrate 0.1g; calcium lactate 0.05g; Cycloserine 0.05g; Trichosanone 0.001g; Vitamin K 1 0.1g; Sterile defibrinated sheep blood 100ml; Distilled water added to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferric ammonium citrate; calcium lactate; , add sterilized D-cycloserine; sterilized vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.
Embodiment 2
[0011] Example 2, the formula is: beef liver powder 10.0g; malt extract powder 5.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; ferric ammonium citrate 0.3g; calcium lactate 0.03g; Cycloserine 0.03g; Trichosanone 0.003g; Vitamin K 1 0.01g; complexin phosphopeptide 0.1g; sterile defibrinated sheep blood 80ml;; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferric ammonium citrate; calcium lactate; ℃, add sterilized D-cycloserine; sterilized vitamin K 1 ; Sterilized Collin Phosphopeptide; Sterile defibrinated sheep blood mixed evenly, sterilized distilled water to 1000ml before packing.
Embodiment 3
[0012] Example 3, the formula is: beef liver powder 5.0g; malt extract powder 3.0g; yeast extract powder 10.0g; sodium chloride 5.0g; agar 20.0g; ferric ammonium citrate 0.2g; calcium lactate 0.03g; Cycloserine 0.01g; Trichosanone 0.001g; Vitamin K 1 0.03g; 0.3g of complexin phosphopeptide; 90ml of sterile defibrinated sheep blood; 250ml of rehmannia root water extract with a concentration of 200g / L; Preparation method Preparation method: (1) Take 50g of rehmannia glutinosa and add water to boil for 45 minutes, filter, take the filtrate, and according to the volume of the filtrate, concentrate or add water to adjust the volume to a concentration of 200g / L to obtain 250ml of rehmannia glutinosa water extract; (2) weigh Take bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferric ammonium citrate; calcium lactate; Sterilize at ℃ for 15 minutes, cool to 45℃, add sterilized D-cycloserine; sterilized colulin phosphopeptide; sterilized vitamin...
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