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Biomark detection method of Alzheimer's disease

A detection method and a biomarker technology, applied in the field of biomedicine, can solve the problem that there is no effective method for the treatment of senile dementia, and achieve the effect of convenient operation

Inactive Publication Date: 2015-08-19
XIANSIDA NANJING BIOTECH CO LTD
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Problems solved by technology

Just because of the uncertainty of its pathogenesis, so far, there is no really effective method for the treatment of senile dementia.

Method used

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  • Biomark detection method of Alzheimer's disease
  • Biomark detection method of Alzheimer's disease
  • Biomark detection method of Alzheimer's disease

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Experimental program
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Embodiment Construction

[0018] Classification of research objects:

[0019] Patient demographics, major clinical and neuropathological data are summarized in Table 1. Clinical diagnosis of cerebrospinal fluid samples were collected from 48 patients with Alzheimer's disease (24 males, 24 females), as well as a group of patients with frontotemporal dementia, vascular dementia, and Lewy body dementia (18 males, 13 females) ).

[0020]

[0021] Table 1 Clinical and neuropathological data

[0022] We used DNA sequencer ABI3130 to obtain N-glycan profiles from asialic acid serum samples of 48 Alzheimer patients and 31 non-Alzheimer dementia patients by DSA-FACE technology, and also analyzed 154 healthy donations. patient's serum samples.

[0023] See figure 1 : shows the oligosaccharide chain fingerprint corresponding to the N-glycan structure, maltooligosaccharides are shown on the top as a sugar quality reference, the glucose units (degree of polymerization) in these structures have been shown, a ...

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Abstract

The invention relates to a biomark detection method of Alzheimer's disease, which comprises the following steps: (1) collecting and processing a serum sample; (2) performing fluorescence marking on an oligosaccharide in the serum sample with a fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) technology through a DNA-based sequencer; (3) measuring content of the oligosaccharide subjected to fluorescence marking in the sample by ionophortic separation and obtaining an oligosaccharide fingerprint spectrum; (4) analyzing the oligosaccharide fingerprint spectrum. The difficult detection of the Alzheimer's disease is solved, the Alzheimer's disease can be quantitatively detected and a pathogenetic degree can be quantitatively evaluated.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a biomarker detection method for Alzheimer's disease. Background technique [0002] Alzheimer's disease can be roughly divided into Alzheimer's disease (Alzheimer's disease, AD) and vascular dementia (vascular dementia, VD) and the mixed type. AD is more common and more studied. The disorder is likely to be a heterogeneous group of disorders, with multiple factors, including biological and psychosocial, contributing to its onset. Judging from the current research, there are more than 30 possible factors and hypotheses for the disease, such as family history, female gender, head trauma, low education level, thyroid disease, maternal age too high or too low, virus infection, etc. [0003] Alzheimer's disease is a worldwide medical and nursing problem. Its pathogenesis is very complex, and the etiology is still unclear, including a variety of hypotheses, such as neurotransmi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6896G01N2800/2821
Inventor 陈翠英
Owner XIANSIDA NANJING BIOTECH CO LTD
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