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Major SNP markers affecting resistance to enterotoxigenic Escherichia coli f41 diarrheal disease in weaned piglets and their application

A technology of Escherichia coli and weaned piglets, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as animal subject research

Active Publication Date: 2018-04-27
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The above-mentioned studies are mainly aimed at pathogens, and have not been conducted on animal subjects from a genetic point of view, and cannot fundamentally solve the problem of piglet diarrhea
Therefore, the current problem is the need to find genes and single nucleotide polymorphisms (SNPs) that affect the traits of piglet diarrhea, in order to establish marker-assisted selection technology to develop enterotoxigenic Escherichia coli F41 diarrhea in weaned piglets. Disease-resistant breeding work to fundamentally solve the problem of piglet diarrhea

Method used

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  • Major SNP markers affecting resistance to enterotoxigenic Escherichia coli f41 diarrheal disease in weaned piglets and their application
  • Major SNP markers affecting resistance to enterotoxigenic Escherichia coli f41 diarrheal disease in weaned piglets and their application
  • Major SNP markers affecting resistance to enterotoxigenic Escherichia coli f41 diarrheal disease in weaned piglets and their application

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Embodiment 1

[0066] The self-constructed pig resource family with white Duroc and Chinese Erhualian pigs as ancestors and used for functional gene mapping was used as experimental animals. 741 individuals of the second generation (F2 generation) of the resource family were bred until they were slaughtered at 240 days old, and the small intestine and jejunum were taken, and the brush border of small intestinal epithelial cells was extracted, and E.coli F41 strain was used to adhere to the brush border of porcine small intestinal epithelial cells. Detection of bacterial adhesion results. More than 20 brush borders were detected for each sample. If at least 2 bacteria adhered to more than 10% of the brush borders, the sample was judged as adherent type. If at least 2 bacteria adhered to the brush borders, less than 10 %, but there are more than 10% of brush borders with 1-2 bacterial adhesion, the sample is judged as weak adhesion type, otherwise it is judged as non-adhesion type. The result...

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Abstract

The present invention relates to a main effect SNP marker affecting the resistance of weaned piglets to enterotoxigenic E. / or main effect SNP marker II; wherein, the site of the main effect SNP marker I is the 513th position of the DNA sequence shown in SEQ ID NO:1, which is a mutation of T and A. The site of the main effect SNP marker II is the 173rd position of the DNA sequence shown in SEQ ID NO: 2, which is a mutation of A and G. The present invention detects the two main-effect SNP markers by means of modern molecular biology techniques, uses marker-assisted selection (MAS) to select favorable allele-type individuals to save seeds, and can significantly improve the resistance to enterotoxigenic Escherichia coli F41 of population weaned piglets The ability to prevent diarrheal diseases greatly reduces piglet mortality.

Description

technical field [0001] The invention belongs to the field of animal molecular biology technology and breeding, and relates to a main effect SNP marker that affects the resistance of weaned piglets to enterotoxigenic Escherichia coli F41 diarrhea disease and its application. Background technique [0002] Infectious diseases of livestock and poultry cause huge economic losses to livestock and poultry production all over the world every year. The losses caused by this account for 17% (1.7 billion pounds) of the annual trade volume in developed countries, and as high as 35%-50% in developing countries. Piglet diarrhea is a common disease in pig production. The mortality rate of piglets caused by diarrhea is 11.5%-29.5%. Among them, the morbidity and mortality caused by E. coli account for 56.2% of the incidence and mortality of pig diarrhea and 24.7%. Escherichia coli disease seriously affects the growth and development of piglets, leading to a decrease in growth efficiency, st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 黄路生任军杨斌晏学明季华员
Owner JIANGXI AGRICULTURAL UNIVERSITY