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Method for preparing monolithic columns

A kind of use, technology of separation column, applied in the field of preparing monolithic column

Inactive Publication Date: 2017-09-08
HELSE STAVANGER HF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So a solution that seems to work perfectly in a vial and even in a 0.1 mm capillary may not work at all in a 0.05 mm ID capillary and lower

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  • Method for preparing monolithic columns
  • Method for preparing monolithic columns
  • Method for preparing monolithic columns

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Embodiment Construction

[0070] As stated above, the present invention relates to methods of preparing monolithic columns, preferably capillary columns or channels in microfabricated devices, which are capable of highly sensitive nanoscale separations of peptides and other molecules, and which preferably have a diameter of 0.05 mm or less. Narrow inner diameter (ID).

[0071] Although the invention is not limited to the internal diameter or material of the column or channel, the following description will largely refer to fused silica capillary columns.

[0072] Before further describing the invention, a general description of monolithic columns and their preparation is given below.

[0073] polymer monolithic column

[0074] Monolithic chromatography stationary phases consist of a single segment of highly porous material that does not contain the interparticle voids typical of packed chromatographic beds. Most of the pores inside the monolith are open, forming an interconnected network of channel...

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Abstract

A method of preparing a separation column or a channel in a microdevice comprising the steps of: providing an unfilled column or channel in a microdevice; activating the inner walls of the column or channel with an activator; a polymerized solution of a mixture of the porogen and at least one polymerization initiator fills the column; and polymerizes the mixture in the column or channel to form a rigid porous monolithic polymer plug. The process is characterized in that the monomer comprises a mixture of divinylbenzene and isodecyl acrylate as main monomers, and that the at least one porogen comprises isobutanol.

Description

[0001] field of invention [0002] The present invention relates to the preparation of columns for liquid chromatography, more particularly polymeric monolithic columns, especially for nanoscale separations of peptides and other molecules. [0003] Background of the invention [0004] Proteomics can be defined as the large-scale analysis of proteins and holds promise for future biomarker discovery in clinical research. In bottom-up proteomics, the proteins in the proteome to be analyzed are identified and their amino acid sequences and post-translational modifications are characterized by proteolytic digestion followed by mass spectrometry (MS) analysis of the resulting peptide. If the number of proteins is large, the corresponding number of peptides is even greater, since many proteins yield more than 10 peptides when cleaved with specific enzymes such as trypsin. Therefore, powerful tools of separation and detection must be applied to successful proteomics experiments. Liq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01D15/02B01J20/285C08F212/36
CPCB01J20/261B01J20/264B01J20/285B01J2220/86B01D15/206G01N2030/567C08J2205/10C08J2325/04G01N30/6095B01D15/08C08F212/36G01N30/60C08F220/1811C08J9/0042
Inventor C.布雷德
Owner HELSE STAVANGER HF