Evaluation methods and phantom samples for optical analysis devices

Abstract

Evaluate the optical system of the optical analysis device easily and with high accuracy. Provided is an evaluation method of an optical analysis device provided with an optical system (A) capable of condensing excitation light B to form a confocal region (C) at a focal position, comprising the steps of: disposing A phantom sample (1) formed by adjacently arranging two or more solid members (2, 3) with different concentrations of fluorescent substances; (1) Move relatively along the arrangement direction of the solid components (2, 3), while irradiating excitation light to the phantom sample (1) through the optical system (A); detect the solid components (2) arranged in the confocal area (C) , the fluorescence generated at 3); and evaluating the optical system (A) based on the detected fluorescence.

Description

technical field [0001] The invention relates to an evaluation method of an optical analysis device and a phantom sample. Background technique [0002] Conventionally, an optical analysis device is known (for example, refer to Patent Document 1.) that can quantitatively observe phases that can be processed by optical analysis techniques including statistical processing such as fluorescence correlation spectroscopy (Fluorescence Correlation Spectroscopy: FCS). The state and characteristics of luminescent particles in an aqueous solution with a lower concentration. [0003] This optical analysis device uses an optical system capable of detecting light from a confocal region (confocal region) in an aqueous solution, such as a confocal microscope or a multiphoton excitation microscope. Then, when the position of the confocal region is moved in the aqueous solution, that is, while scanning the aqueous solution through the confocal region, it is detected that light-emitting partic...

AI Technical Summary

Problems solved by technology

[0007] At this time, when it is confirmed that the number of peaks obtained for a certain predetermined concentration is less than the number estimated from the optical system, or the height of the pulse train of photons forming the peak is small, that is, the intensity of fluorescence is small, etc., these Indicates that the state of the optical system is inaccurate

Images