Infertility joint detection kit and detection method thereof

A combined detection, infertility technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem of inability to achieve single-part multi-item detection, negative control non-specific color rendering, slow reagent reaction, etc. problem, to achieve the effect of good sealing effect, satisfying clinical use and high sensitivity

Inactive Publication Date: 2015-10-14
厦门拜尔杰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a combined infertility detection kit and its detection method to solve the problem that existing infertility detection products cannot detect multiple items for a single person, have low sensitivity, troublesome operation, and non-specific negative control Disadvantages such as strong color development and slow reaction of reagents

Method used

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  • Infertility joint detection kit and detection method thereof

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Effect test

Embodiment 1

[0025] A combined infertility detection kit containing 20 detection cards, specifically including the following four reagents: 10mL of sample diluent, 5mL of enzyme conjugate, 10mL of washing solution and 5mL of substrate. Among them, the sample diluent includes 0.46% sodium dihydrogen phosphate, 0.58% disodium hydrogen phosphate dodecahydrate, 2% sodium chloride, 5% newborn calf serum, 0.2% lithium chloride, 0.2% casein, 0.1% Carboxymethylcellulose sodium salt, 0.04% ethylenediaminetetraacetic acid, 0.1% Proclin-300, the rest is deionized water or distilled water; enzyme conjugates include 0.46% tris, 1% bovine serum albumin, 0.2% lithium chloride, 0.8% sodium chloride, 0.1% Tween 80, 0.2% polyethylene glycol, 0.04% ethylenediaminetetraacetic acid, 0.5% sodium p-hydroxybenzoate, 0.05% HRP-labeled goat anti-human IgG, 0.1% Proclin-300, the rest is deionized water or distilled water; washing solution includes 0.46% sodium dihydrogen phosphate, 0.58% disodium hydrogen phosphate ...

Embodiment 2

[0027] A combined test kit for infertility, containing 10 test cards, specifically including the following four reagents: 5 mL of sample diluent, 3 mL of enzyme conjugate, 5 mL of washing solution and 3 mL of substrate. Among them, the sample diluent includes 0.46% sodium dihydrogen phosphate, 0.58% disodium hydrogen phosphate dodecahydrate, 2% sodium chloride, 5% newborn calf serum, 0.2% lithium chloride, 0.2% casein, 0.1% Carboxymethylcellulose sodium salt, 0.04% ethylenediaminetetraacetic acid, 0.1% Proclin-300, the rest is deionized water or distilled water; enzyme conjugates include 0.46% tris, 1% bovine serum albumin, 0.2% lithium chloride, 0.8% sodium chloride, 0.1% Tween 80, 0.2% polyethylene glycol, 0.04% ethylenediaminetetraacetic acid, 0.5% sodium p-hydroxybenzoate, 0.05% HRP-labeled goat anti-human IgG, 0.1% Proclin-300, the rest is deionized water or distilled water; washing solution includes 0.46% sodium dihydrogen phosphate, 0.58% disodium hydrogen phosphate dod...

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Abstract

The invention discloses an infertility joint detection kit which comprises four reagents including a sample diluent, an enzyme combination, flushing fluid and a substrate. The invention further discloses a detection method for the joint detection kit. The detection method comprises the following steps: balancing the detection kit to room temperature, treating a sample, rinsing the inner film surface of a card window, adding the sample and the flushing fluid, adding the enzyme combination, performing development, ending the operation and the like. The infertility joint detection kit can be used for simultaneously detecting multiple persons in multiple channels, and the sensitivity can reach the detection level of an ELISA product and is much higher than a colloid product; meanwhile, the detection time of the detection kit is close to that of colloid and much shorter than that of ELISA, and operation of a professional is not needed; the infertility joint detection kit is convenient and efficient and can well meet clinical use.

Description

technical field [0001] The invention relates to a detection kit, more specifically to a combined detection kit for infertility and a detection method thereof. Background technique [0002] At present, the common products used for infertility detection in the market mainly include colloidal gold detection, ELISA detection and other detection forms. Colloidal gold detection is to bind the antigen or antibody on the nitrocellulose membrane, and dry the labeled antibody or antigen colloidal gold on the binding pad. If the sample contains specific antibody or antigen, it can be combined with the antigen or antibody on the colloidal gold, And they are chromatographed in the direction of the absorbent paper together. When the chromatogram reaches the detection line, it will combine with the antigen or antibody on the detection line, and finally judge the positive or negative by the color. Colloidal gold is mainly used for single-item single-item testing (that is, only one item...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/50G01N2800/367
Inventor 陈隆玉史正文
Owner 厦门拜尔杰生物科技有限公司
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