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Bactericides and their applications

A fungicide, listeria technology, applied in the direction of fungicide, application, biocide, etc., can solve the problems of carcinogen formation, poor sterilization effect, complex surface structure of vegetables, etc., and achieve the effect of easy preparation

Active Publication Date: 2017-12-08
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The physical sterilization method has the advantage of no pollution, but it requires special equipment and protection requirements, and it needs to go to a special institution for sterilization. Although ultraviolet sterilization can be used in factory production, the sterilization effect is not good due to the complex surface structure of vegetables.
[0004] 2. The chemical sterilization method has the advantages of convenient application and can be widely used in processing plants, but chlorine-containing agents such as sodium hypochlorite are easy to react with organic matter and form carcinogens; chlorine dioxide is unstable and has a strong smell, so it has become a challenge to find alternatives to sodium hypochlorite The Direction of Controlling Pathogenic Microorganisms in Postharvest Vegetables
Surfactants have the characteristics of safety, but there is a problem of poor bactericidal effect, which needs further development

Method used

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  • Bactericides and their applications
  • Bactericides and their applications
  • Bactericides and their applications

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, surfactant thiamine dilauryl sulfate (TDS) bactericidal effect

[0040] Killing effect of TDS on E.coli O157:H7.

[0041] Methods: TDS mother solution was prepared with 100% ethanol, and then diluted with sterile water to obtain TDS working solution with a concentration of 0.1%, 0.5% and 1.0%, and the ethanol concentration was about 2%, 10% and 20%, respectively. Take 10 μL of coli O157:H7 (concentration is about 9 log CFU / mL) with a pipette gun, respectively add to 990 μL of the TDS solution of different concentrations above, mix well, place at room temperature for 1 min and 24 hrs, then draw 100 μL to coat the plate ( Tryptone soybean agar medium, TSA), cultivated at 37°C for 24h, counted, and the experimental value is the average value of three repetitions.

[0042] The obtained results are shown in Table 1.

[0043] Table 1. The effect of TDS on the quantity of E.coli O157:H7

[0044]

[0045] Note: The initial quantity of E.coli O157:H7 is 7.14±...

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Abstract

The invention discloses a fungicide and its application. The fungicide solution is a fungicide solution with thiamine dilauryl sulfate, ethanol and water as active ingredients. The composition of each component in the fungicide solution is specifically as follows: the volume percent concentration of thiamine dilauryl sulfate is 0.1%-1.0%, specifically 0.1%-0.5% or 0.5%-1.0%. The volume percent concentration of ethanol is 5%-20%; the balance is water. Treating with this fungicide for 1 minute can achieve the effect of reducing at least 7 log CFU / mL of E.coli O157:H7, Salmonella and Listeria monocytogenes. If it is used to wash vegetables such as spinach, the fungicide should be mixed according to the volume / mass ratio of 10L:1Kg Mix with vegetables, shake and wash at 1200rpm / min for 1 minute, which can achieve at least the effect of reducing E.coli O157:H74log CFU / leaf.

Description

technical field [0001] The invention relates to a fungicide and its application. Background technique [0002] Vegetables are contaminated with E.coli O157:H7 during the entire supply chain from pre-harvest to post-harvest. Consumers eat vegetables contaminated with E.coli O157:H7, which can easily cause diarrhea, internal bleeding and other health-threatening diseases. cause death. The methods currently used to control vegetable E.coli O157:H7 are mainly physical sterilization methods and chemical sterilization methods, wherein physical sterilization methods include methods such as irradiation, electron beams, and ultraviolet rays, and chemical methods include bactericides such as sodium hypochlorite, electrolyzed water (containing Sodium hypochlorite), chlorine dioxide and tensio-active agent etc., they have following defect respectively: [0003] 1. The physical sterilization method has the advantage of no pollution, but requires special equipment and protection require...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/30A01N43/78A01P1/00A01P3/00C11D1/88C11D3/20C11D3/48
Inventor 张学杰
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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