Bactericides and their applications
A fungicide, listeria technology, applied in the direction of fungicide, application, biocide, etc., can solve the problems of carcinogen formation, poor sterilization effect, complex surface structure of vegetables, etc., and achieve the effect of easy preparation
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[0039] Embodiment 1, surfactant thiamine dilauryl sulfate (TDS) bactericidal effect
[0040] Killing effect of TDS on E.coli O157:H7.
[0041] Methods: TDS mother solution was prepared with 100% ethanol, and then diluted with sterile water to obtain TDS working solution with a concentration of 0.1%, 0.5% and 1.0%, and the ethanol concentration was about 2%, 10% and 20%, respectively. Take 10 μL of coli O157:H7 (concentration is about 9 log CFU / mL) with a pipette gun, respectively add to 990 μL of the TDS solution of different concentrations above, mix well, place at room temperature for 1 min and 24 hrs, then draw 100 μL to coat the plate ( Tryptone soybean agar medium, TSA), cultivated at 37°C for 24h, counted, and the experimental value is the average value of three repetitions.
[0042] The obtained results are shown in Table 1.
[0043] Table 1. The effect of TDS on the quantity of E.coli O157:H7
[0044]
[0045] Note: The initial quantity of E.coli O157:H7 is 7.14±...
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