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Tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step

A technology of Polygonatum chinensis and seeds, which is applied in the field of tissue culture for seedlings of Polygonatum chinensis seeds in one step, can solve the problems of high production cost, high technical difficulty, long time period and the like, and achieve the effects of low cost, shortened germination period and developed root system.

Active Publication Date: 2015-11-18
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects of complex procedures, high technical difficulty, long time period and high production cost in the prior art, and provide a new method for tissue culture of Polygonatum yunnanensis seedlings in one step

Method used

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  • Tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step
  • Tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step
  • Tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step

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Experimental program
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Embodiment 1

[0032] This embodiment is carried out in the laboratory of Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming City, Yunnan Province.

[0033] (1) Selection of Polygonatum Polygonatum Fruit

[0034] In October, 80% of mature fresh and pest-free Polygonatum yunnanensis fruits were selected and stored in a refrigerator at 4°C for 7 days.

[0035] (2) Treatment of Rhizoma Polygonatum Seeds

[0036] Peel off the peel of the selected Polygonatum yunnanensis fruit, take out the seeds and put them in a beaker, disinfect them with 75% alcohol by volume for 1 min, wash them twice with sterile water, transfer them to a sterile workbench, and wash them with 0.1% HgCl 2 After soaking in the solution for 14 minutes, rinse with sterile water 3 times;

[0037] (3) One-step seedling cultivation of Polygonatum yunnanensis seeds

[0038] The Rhizoma Polygonatum seeds treated in step (2) were inoculated in a culture bottle filled with MS+BA0.5mg / L medium, and the c...

Embodiment 2

[0042] Embodiment 2 is the same as Embodiment 1 except the following parameters are different, and will not be repeated here.

[0043] (2) Treatment of Rhizoma Polygonatum Seeds

[0044] The taken out seeds were sterilized with 75% alcohol for 1 min and rinsed with sterile water for 3 times; transferred to a sterile workbench and washed with 0.1% HgCl 2Soak in the solution for 15 minutes, rinse 4 times with sterile water;

[0045] (3) One-step seedling cultivation of Polygonatum yunnanensis seeds

[0046] The Rhizoma Polygonatum Seeds processed through step (2) are inoculated in the culture bottle filled with MS+BA0.5mg / L medium, the density of Rhizoma Rhizoma Rhizoma Rhizoma in the culture bottle is 10 or 15 grains / bottle, and the seed density is too dense It is not conducive to the growth of plants, and if it is too thin, the medium will be wasted. The diameter of culture bottle is 7cm, height is 11cm, is 23 ℃ at culture temperature, and light time is 14h / day, and light ...

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Abstract

The invention discloses a tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step. The method comprises steps as follows: selecting Polygonatum kingianum fruits, treating Polygonatum kingianum seeds, culturing the Polygonatum kingianum seeds into the seedlings in one step and performing seedling exercise and transplantation. Medium-well mature fruits are selected and placed in a refrigerator at the temperature of 4 DEG C for 7 days; a culture bottle containing an MS+BA 0.5 mg / L culture medium is inoculated with the treated seeds, the seeds are cultured under the conditions that the temperature is 25 DEG C plus / minus 2 DEG C, the illumination time is 12-14 h / day and the illumination intensity is 150 lx until the Polygonatum kingianum seeds send forth two true leaves, and the seeds are continuously cultured under the conditions that the culture bottle is placed at the culture temperature of 25 DEG C plus / minus 2 DEG C, the illumination time is 12 h / day and the illumination intensity is 1500 lx until rooting. With the adoption of the method, only 90-99 days are taken from inoculation for the seeds to one-step culture of the seedlings, the germination period is shortened to 1 month from two winters, the propagation cycle is greatly shortened, the seed germination rate is in a range of 90%-95%, and the excised seedling survival rate is 100%.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, and in particular relates to a tissue culture method for one-step seedling formation of Polygonatum yunnanensis seeds. Background technique [0002] Polygonatum kingianum (Polygonatum kingianum Coll. et Hemsl.) is a herb of the genus Polygonatum kingianum Coll.et Hemsl. in the family Liliaceae, and is one of the three basic plants of Polygonatum kingianum commonly used in traditional Chinese medicine. Polygonatum, as a commonly used traditional Chinese medicine, has a history of more than 2,000 years in my country. Its nature and taste are sweet and flat, and it enters the lung, spleen and kidney meridians. Efficacy Buzhongyiqi, moisten the heart and lungs, strengthen the bones and muscles. Control cold and heat due to deficiency, tuberculosis and hemoptysis, lack of food after illness, weakness of muscles and bones, rheumatism pain, wind leprosy and ringworm disease...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 季鹏章张智慧王家金石亚娜刘大会朱新焰王丽马聪吉
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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