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Application of coniferyl ferulate to preparing antidepressant drugs

A technology of coniferyl ferulate and anti-depression, applied in the field of medicine, to achieve the effect of shortening immobility time, strong action, and wide sources

Active Publication Date: 2015-11-18
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the application of coniferyl ferulate in the preparation of drugs for the prevention or treatment of depression

Method used

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  • Application of coniferyl ferulate to preparing antidepressant drugs
  • Application of coniferyl ferulate to preparing antidepressant drugs
  • Application of coniferyl ferulate to preparing antidepressant drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Protective activity of coniferyl ferulate against corticosterone and glutamate damage to PC12 cells respectively

[0019] 1. Cell culture

[0020] PC12 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. The culture system used RPMI-1640 medium containing 5% fetal bovine serum and 10% horse serum at 37°C, 5% CO 2 cultured in a cell culture incubator. Coat culture flasks and culture dishes with poly-L-lysine (0.01mg / m1) before culturing, and change the medium every 3 days.

[0021] 2. Experimental steps

[0022] The present invention adopts the MTT method to evaluate the protective activity of coniferyl ferulate on PC12 cells damaged by corticosterone and glutamic acid respectively. After PC12 cells were digested with trypsin, the number of cells per milliliter was 2×10 5 The suspension was inoculated in a 96-well culture plate, 100 μL per well, and placed in 37°C 5% CO 2 Incubator culture. After 24 hours, discard the cell...

Embodiment 2

[0028] Example 2 In vivo pharmacodynamics experiment of coniferyl ferulate antidepressant

[0029] 1.1 Experimental materials and grouping

[0030] Male ICR mice, weighing 18-20 g, were provided by Beijing Weitong Lihua Animal Experiment Center. Each 10 birds / cage was raised, free to eat and drink, room temperature (22±2)°C, natural light. Normal mice were randomly divided into 5 groups according to body weight, 12 in every group, i.e. blank control group, positive drug fluoxetine group (10mg / kg), coniferyl ferulate high (40mg / kg), middle (20mg / kg ), low (10mg / kg) dose group. Each group was administered intragastrically at 0.2mL / 10g body weight for 7 days.

[0031] 1.2 Experimental method

[0032] 1.2.1 Mouse tail suspension test

[0033] After 1 hour of administration on the 7th day, the tail end of a single mouse (2 cm away from the tail end) was glued to the upper part of the carton with adhesive tape, making it in an upside-down state, with the head 15 cm away from th...

Embodiment 3

[0046] Embodiment 3. The preparation of coniferyl ferulic acid soft capsule

[0047] Get coniferyl ferulate 20g, vegetable oil 180g, stir, fully mix, set aside as content. Take appropriate amount of glycerin, gelatin and water (1:3:3 ratio), add appropriate amount of water to the gelatin to make it fully absorb water and swell, mix glycerin and the rest of the water, put it into a plastic bucket and heat to 70-80°C , add an appropriate amount of expanded gelatin and propyl p-hydroxybenzoate, stir, and use it as a capsule material for later use. The contents and capsule material are pressed into soft capsules on a soft capsule machine, dried and shaped to obtain coniferyl ferulate soft capsules.

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Abstract

The invention provides application of coniferyl ferulate to preparing antidepressant drugs, and relates to the field of medicine. The application has the advantages that as proved by in-vitro activity tests, the coniferyl ferulate is excellent in protection activity for PC12 cells injured by corticosterone and glutamic acid; as shown by results of in-vivo tests, the immobility time of mice in forced swimming and tail suspension tests can be obviously shortened by the coniferyl ferulate; as proved by tests, obvious antidepressant effects can be realized by the coniferyl ferulate, and accordingly the coniferyl ferulate can be used for preparing the drugs for preventing and treating depression.

Description

Technical field: [0001] The invention relates to the field of medicine, in particular to the application of coniferyl ferulate in the preparation of antidepressant drugs. Background technique: [0002] Depression is characterized by persistent low mood and cognitive dysfunction, including restlessness, anhedonia, sleep disturbance, guilt and repeated suicidal thoughts, etc. Disabling rate and high fatality rate and so on. The World Health Organization reports that more than 350 million people worldwide suffer from depression, and by 2020 depression may become the second leading disease after heart disease. With the rapid development of social economy, the pace of life is accelerated, people's pressure increases, and various emotional impacts increase, resulting in an increase in the incidence of depression year by year. Depression is called "the epidemic of the 21st century", and its harm has attracted more and more attention from the medical and health circles. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P25/24
Inventor 周玉枝宫文霞秦雪梅张丽增高晓霞田俊生
Owner SHANXI UNIV
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