A quantitative detection method for exosome miRNA

A quantitative detection and exosome technology, applied in the field of quantitative detection of exosome miRNA, can solve the problems of high error rate of preference, influence on detection, and few miRNAs, and achieve the effect of correcting related sequencing errors and avoiding preference
CN105063209BActive Publication Date: 2017-12-12BEIJING GENEPLUS TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING GENEPLUS TECH
Publication Date
2017-12-12

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Abstract

The invention provides a quantitative detection method of exosome miRNA (micro ribonucleic acid). The quantitative detection method comprises steps as follows: exosome total RNA extraction, connection of 3'-terminal and 5'-terminal specificity unique tagged connectors, reverse transcription synthesis of cDNA (complementary deoxyribonucleic acid), PCR (polymerase chain reaction) amplification, screening, purification and sequencing of a miRNA library on a computer, information analysis of an error correction algorithm and the like. According to the quantitative detection method, all original templates are distinguished on the basis of marks of the specificity unique tagged connectors, preference of molecules is avoided during amplification, miRNA molecules with the copy number lower than 10 can be detected precisely, the specificity is high, and the method can be used for detection of tumor derived exsome miRNA in the fields of neurodegenerative diseases, cardiovascular diseases, reproductive health and the like, can provide a basis for early screening of diseases, can also be applied to the field of precise quantitative detection of other small RNA and has a wide application prospect and high market value.
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Description

technical field

[0001] The invention belongs to the technical field of high-throughput detection of small molecule RNA, and in particular relates to a quantitative detection method of exosome miRNA. Background technique

[0002] Exosome (Exosome) is a kind of membranous vesicle secreted outside the cell after late endosome (also known as multivesicular body) fuses with the cell membrane. Under the electron microscope, its appearance is a characteristic "disk". Shaped small bodies, with a diameter between 40-100nm, are secreted by living cells and can be secreted by almost all types of cells. The nucleic acid, protein, and lipid components carried by them have the specificity of their source cells and participate in the regulation of many Important cell physiological activities, such as immune response, apoptosis, angiogenesis, inflammatory response, coagulation and other processes, so it has important research significance in related diseases, including neurodegenerative dis...

Claims

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