Molecular-specific marker primers and methods for distinguishing Grass coral and 3 kinds of confounding products

Abstract

The invention relates to a molecular-specific marker primer and a method for distinguishing coral coral from three kinds of obfuscated products, and belongs to the field of biotechnology. Construct a multiplex PCR system: the total amount is 20 μL, extract 10 ng of DNA from plants as an amplification template, add 1×TransStart TopTaq DNA polymerase, 2 μL 10×TransStart TopTaq Buffer, dNTP 2.5mM, 0.2 μM downstream primer trnL‑Ff And the three upstream specific primers SgF, CspF and CsF are 0.1 μM each, and the rest are sterile water. This method is based on the genetic background for molecular identification, and is not affected by external factors such as the place of origin of the medicinal material. Compared with the chemical method, it is more accurate, simpler and more convenient.

Description

technical field

[0001] The invention relates to a molecular-specific marker primer and a method for distinguishing coral coral from three kinds of obfuscated products, and belongs to the field of biotechnology. Background technique

[0002] Sarcandra glabra (Thunb.) Nakai, commonly known as sarcandra, is usually used to treat tinea versicolor, purpura, rheumatic joint pain, and trauma. Modern studies have shown that it has pharmacological effects such as treating cancer, anti-tumor, anti-inflammation, anti-virus and non-specific immune enhancement. However, the germplasm resources of wild plants have been reduced in recent years. Because the appearance of Chrysanthemum praecox and Chrysanthemum broadleaf is very similar to that of Grass coral, it is easy to be confused with Grass coral. However, they have different properties and medicinal value, and these easily mixed plants also have corresponding hepatotoxic effects, and have been confirmed to have significant hepatotoxi...

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