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Methods and kits for identifying microorganisms in a sample

A technology of microorganisms and samples, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve problems such as less microorganisms

Active Publication Date: 2015-11-25
GENEWIZ INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, only a few microorganisms can be successfully cultured in nature, and second, the sequencing conditions are also facing challenges

Method used

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  • Methods and kits for identifying microorganisms in a sample
  • Methods and kits for identifying microorganisms in a sample
  • Methods and kits for identifying microorganisms in a sample

Examples

Experimental program
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Embodiment Construction

[0168] Selection of PCR primers

[0169] The inventor selected primers from hundreds of 16SDNA primers reported in the literature, and improved them for metagenome analysis. 22 primers were selected for improvement.

[0170] Improved primers increase the diversity and coverage of detected microbial species

[0171] The inventor hopes that compared with the existing primers, the improved primers can identify more microorganisms and more types. In the existing 16SDNA sequence identification, the primers used for potential conserved sequences are designed with few repeating regions. The prior art A method for reviewing and merging sequence repeat regions used in the identification of single conserved sequences in overlapping sequences. (see, for example, Wang et al., PLOSONE 4:e7401 (2009)). No previous literature suggested the use of multiple sets of coincident primers or multiple frame shifted amplicons in 16S analysis. However, the inventors speculate that the simultaneous ...

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Abstract

Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.

Description

[0001] Inclusion of sequence listing [0002] The sequence listing is the 30587_ST25.txt file in the ASCII folder, with a size of 19KB, created on April 24, 2014, submitted to the US Patent and Trademark Office through EFS-Web, and incorporated as a reference in this patent. Background technique [0003] Globally nearly 10 30 Microbiome genomes, in different environments and host environments, the diversity between and within the microbiome is well known (Huse et al., PLoS Genetics4 (11): e1000255 (2008)). Because of the complex diversity, the determination of the microbiome The composition of is a challenge for the existing technology. First of all, only a few microorganisms in nature can be successfully cultured, and secondly, sequencing conditions are also facing challenges. The diverse composition of microorganisms in a microbiome and the homology between them make it difficult to identify their species. [0004] The prokaryotic ribosome is composed of two subunits: a 3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12N15/11C12Q1/04C12Q1/68C12Q1/689C12Q2600/16
Inventor 黄隽周祯祯段海蓉吴昕李世红
Owner GENEWIZ INC