A kind of oil emulsion vaccine of cattle and sheep foot rot and preparation method thereof

Abstract

The invention provides an oil emulsion vaccine for cattle and sheep foot rot and a preparation method thereof. The oil emulsion vaccine is prepared from an oil phase and a water phase in parts by weight of (1.5-3):1 by adding a 0.01% thimerosal solution, wherein the oil phase is prepared from autoclaved medicinal white oil, 10% Span-80 white oil mixed liquor and 10% mannitol monooleate white oil mixed liquor according to the ratio of (1.8-2.2):1:1; the water phase is prepared by dissolving 4-7% sucrose ester into a water solution containing 10% tween-80 and 10% polyoxyethylene castor oil derivatives and then mixing with an antigen solution according to the ratio of 1:(2.5-3); and the antigen solution is a bacterial solution obtained by carrying out subculture on fusobacterium necrophorum through a fusobacterium necrophorum subculture medium, carrying out amplification culture through a fusobacterium necrophorum amplification culture medium and then carrying out concentration and inactivation, wherein the bacterial count of the bacterial solution per milliliter is not lower than 4*10<10> CFU. The invention also provides the preparation method of the oil emulsion vaccine. The oil emulsion vaccine provided by the invention has the characteristics of low viscosity, good stability, good immune effect, small clinical adverse reaction and low cost.

Description

technical field [0001] The invention relates to a vaccine for foot rot of cattle and sheep and a preparation method thereof, in particular to an oil emulsion vaccine for foot rot of cattle and sheep and a preparation method thereof. Background technique [0002] Foot rot is a common disease of ruminants. In my country, the incidence of foot rot is relatively low in the northeast, northwest, Tibet, and Inner Mongolia. In other areas, especially in the south, dairy cows, goats, sheep, etc. Foot rot is widespread, and its incidence rate is as high as 8% to 20%, or even 30% to 50%. Due to unscientific feeding management, poor environmental conditions, postpartum diseases, genetics and other factors, dairy cows and sheep suffer from hoof cracks, hoof leaks, sole or heel ulcers, etc., which reduce the resistance of the hooves, eventually causing pathogenic bacteria infections and aggravating the hooves. internal damage. Moreover, the cure rate of cattle and sheep foot rot is low,...

AI Technical Summary

Problems solved by technology

In the production process of traditional oil emulsion vaccines, Span-80 is added to white oil as the oil phase, and Tween-80 and aluminum stearate are added to the aqueous antigen solution as the water phase. The oil emulsion vaccine obtained by mixing and emulsifying the two has high stability. After immunization, a high level of persistent immune antibodies can be obtained; however, the oil emulsion vaccine has a high viscosity, and the stress response caused by immunization injection is relatively large, which may form aseptic suppurative foci, swelling, induration, or formation of Granuloma, poor immune effect; at the same time, it also has a negative impact on the quality of animal meat products

It can be seen that aluminum stearate content, oil phase, water phase, oil phase water phase ratio, shearing time, and the quality of self-oil are important factors affecting the viscosity of oil emulsion vaccines. Oil emulsion vaccines have difficulties in cultivating a large number of bacterial strains, Disadvantages such as immune effects and adverse side effects

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