Bjerkandera sp. Gause 15 for controlling vegetable root diseases and preparation thereof

A technology for root diseases and tobacco pipe bacteria, applied in the direction of fungicides, biocides, fungi, etc., to achieve good development and application prospects, easy industrial production, and low pollution effects

Active Publication Date: 2015-12-02
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the suppression of pathogenic bacteria in crops by the fungus

Method used

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  • Bjerkandera sp. Gause 15 for controlling vegetable root diseases and preparation thereof
  • Bjerkandera sp. Gause 15 for controlling vegetable root diseases and preparation thereof
  • Bjerkandera sp. Gause 15 for controlling vegetable root diseases and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the preparation of Gao's No. 15 bacterial strain fermented liquid

[0040] (1) Medium:

[0041] Gore's medium: 1 g KNO 3 , 0.5 g K 2 HPO 4 , 0.5 g MgSO 4 ·7H 2 O, 0.5 g NaCl, 0.01 g FeSO 4 ·7H 2 O, 20g agar or 13g agar powder, pH=7.4-7.6, 1000ml water.

[0042] (2) Strain culture:

[0043] Scrape a small amount of bacterial liquid from the surface of the preserved strains, streak on the Gowell medium plate, incubate at 30°C for 24 hours, pick a single colony into 2 ml of Gowell medium, 200 rpm, and incubate at 30°C for 24 hours , and then inserted into a 250-mL Erlenmeyer flask with a medium filling volume of 80 mL at a ratio of 10%, cultured at 30° C. for 48 hours at 200 rpm.

[0044] (3) Expand fermentation:

[0045] Inoculate the strain solution in a 50-liter fermenter with a 0.5% inoculation ratio, and feed 35 liters of fermentation culture material in a 50-liter fully automatic fermenter. The fermentation conditions are: temperature 30°C, 12...

Embodiment 2

[0046] Embodiment 2: strain identification and preservation

[0047] 2.1 Morphological characteristics

[0048] Morphological identification: Streak the Gaoshi No. 15 strain on the Gaoshi medium plate, and observe the morphology after culturing at 30°C for 48 hours. After 48 hours of shaking culture in Gaoke's liquid medium, the shape, size, spore formation and flagella growth mode of the bacteria were observed.

[0049] Observation results: Opaque milky white radial colonies are formed on Gore's medium, the surface is thin and unevenly distributed. The mycelium is thin, short and easy to break.

[0050] 2.2 Identification and preservation

[0051] Molecular identification: The DNA gene of Gaoshi 15 was sequenced by Wuhan Aoke Dingsheng Biotechnology Co., Ltd. The sequencing primer was: ITS1, and a gene fragment with a length of 266bp was obtained.

[0052] Comparison of the gene sequence with the published gene sequence on the NCBI website (www.ncbi..nlm.nih) shows that t...

Embodiment 3

[0056] Embodiment 3: the preparation process of Gaoshi No. 15 wettable powder inoculum

[0057] The fermented fermented body is processed into wettable powder according to the following method: firstly carry out the archaic fermentation culture of the thalline (same as expanded fermentation). Then filter through plate frame, dry and pulverize to make wettable powder. The specific production process is as follows:

[0058] Plate and frame filtration: Press the material from the storage tank into the plate and frame with a pressure of 10 kg / cm2, and filter through No. 3 gauze. The pressure should be adjusted at any time so that the content of bacteria in the bacteria does not exceed 20 million / ml.

[0059] Drying: Ventilate and dry in a drying room with a temperature below 60°C to a moisture content of 5-8%.

[0060] Pulverization: The output temperature should not exceed 70°C during pulverization to prevent the inactivation of bacteria.

[0061] Determination of finished pro...

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Abstract

The invention provides bjerkandera sp. Gause 15 and a fungicide prepared from bjerkandera sp. Gause 15. Bjerkandera sp. Gause 15 is collected in the China Center for Type Culture Collection (CCTCC), with collection number of CCTCC NO:M2015351. The fungicide is granular or is wettable powder. The fungicide prepared from bjerkandera sp. Gause 15 provided by the invention has obvious bacteriostatic activities towards Fusarium spp., Sclerotium rolfsii Sacc, Rhizoctonia cerealis Vander Hoeven and Pseudomonas solanacearum Smith. The granular fungicide or wettable powder prepared from the strain can be used for controlling tomato root rot, tomato damping off, tomato bacterial wilt, konjac root rot, konjac southern blight, green pepper root rot, green pepper bacterial wilt, green pepper damping off, watermelon root rot, watermelon bacterial wilt and watermelon damping off.

Description

technical field [0001] The invention relates to a porous tobacco fungus (Bjerkanderasp.), a fungal agent produced by the porous tobacco fungus and the application of preventing and treating vegetable root diseases. The bacterial strain has been preserved in the China Center for Type Culture Collection, the preservation number is CCTCC: M2015351, the bacterial strain is named Bjerkanderasp. Gaoshi No. 15, and belongs to the field of biotechnology. Background technique [0002] With the global attention to resources and the environment, and the development of harmless agriculture, the trend of using living organisms as pharmaceuticals has been increasing in recent years. Developed countries in the world are actively looking for microbial resources that can be developed and utilized. Fusarium, Rhizoctonia solani, Sclerotinia and Pseudomonas solanacearum cause vegetable root diseases, especially bacterial wilt is the most harmful root disease in my country's vegetable production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 汪华
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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