A kind of detection method of target molecular concentration

Abstract

A method of detecting a target molecule concentration comprises the following steps: (1) coupling a first molecular probe to a surface of a magnetic bead to form a magnetic bead probe, and coupling second molecular probes to surfaces of target particles to form target probes; (2) mixing the magnetic bead probe with a target molecule solution so as to capture a target particles at a surface of the magnetic bead probe; (3) adding the target probes, combining the target molecule with the second molecular probes, and forming a magnetic bead probe-target molecule-target probe composite; (4) removing an unreacted target probe; (5) adding an eluent, dissociating a structure of the magnetic bead probe-target molecule-target probe composite into the magnetic bead probe, target molecule, and target probe; (6) extracting a solution obtained from Step (5) to prepare a specimen on a microscope slide, counting and calculating, using a microscope, a total number of target probes, and calculating a target molecule concentration accordingly. The detection method provides increased reliability and sensibility.

Description

technical field [0001] The invention relates to a biological detection method, in particular to a detection method for the concentration of a target molecule. Background technique [0002] Highly sensitive biological detection is of great significance in the diagnosis and treatment of diseases (especially in the early stage of disease development), detection of bacteria and viruses, and clinical basic research. At present, detection methods based on fluorescent labels are the main means to achieve higher sensitivity detection. In typical commercial label detection technologies such as biochip (chip array), liquid phase biochip (suspension array), and enzyme-linked immunomagnification (ELISA), the target molecule is first immobilized on the surface of the substrate on which the probe molecule is functionalized. Afterwards, fluorescent molecules or enzymes with strong optical signals mark the immobilized target molecules, and the measured optical signal intensity has a positi...

AI Technical Summary

Problems solved by technology

Ordinary single-molecule detection techniques often require expensive instrument support, such as confocal fluorescence microscopes equipped with single-photon detection capabilities, total internal reflection fluorescence microscopes, etc., which are often too expensive in cost and difficult to apply to practical high-sensitivity detection

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