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A device and method for extracting apoplast juice from plant fruit tissue

An apoplast and fruit technology, which is applied in the field of plant fruit tissue apoplast juice extraction, can solve the problem of no plant fruit apoplast juice extraction method and the like, and achieves the effects of high purity, high efficiency and avoiding pollution

Active Publication Date: 2018-09-14
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is still no report on the extraction method of plant fruit exosome juice

Method used

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  • A device and method for extracting apoplast juice from plant fruit tissue
  • A device and method for extracting apoplast juice from plant fruit tissue
  • A device and method for extracting apoplast juice from plant fruit tissue

Examples

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Effect test

Embodiment 1

[0055] Present embodiment is the method for separating apoplast juice from strawberry fruit tissue:

[0056] 1. Separation of apoplast juice

[0057] 1. Take 3 strawberry fruits 25 days after flowering. In order to reduce the change of the exoplasmic components of the fruit, the subsequent operations must be carried out at 4°C.

[0058] 2. Cut the fruit into 0.1cm with a sterilized blade 3 Rinse three times with deionized water, then put it into a 100ml small beaker, submerge it in deionized water, and filter it in a vacuum pump for 5 minutes.

[0059] 3. Immediately after the sample is taken out, blot the water on the surface of the sample with absorbent paper, and put it into a self-made centrifuge device.

[0060] 4. Set the speed of the centrifuge to 1500g, centrifuge for 6 minutes, then use a pipette to suck up the extract and discard it.

[0061] 5. Gradient centrifugation, centrifuging for 6 minutes each time, and gradually increasing the centrifugal force from 1500g...

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Abstract

The invention discloses a device and method for extracting apoplast juice from plant fruit tissue. The device comprises a centrifugal bottle, centrifugal screens and a movable screen plate. The centrifugal bottle is of a cylindrical structure. the centrifugal screens are of a cylindrical structure and are arranged in the centrifugal bottle, the movable screen plate is arranged in the centrifugal screens in a clamped mode to divide the centrifugal screens into two layers, the outer wall of the centrifugal screen on the upper layer is provided with a plurality of through holes, and a plurality of through holes are formed in the bottoms of the centrifugal screens. By means of the device and the method, plant apoplast juice can be extracted from plant fruit tissue, the device and the method can be more widely applied to extraction of plant apoplast juice, and thus significance is achieved on researching the growth and development of plants with participation of apoplast matter, signal transduction, stress responses and other mechanisms.

Description

technical field [0001] The invention relates to the field of extracting apoplast juice from plant fruit tissue, in particular to a device and method for extracting apoplast juice from plant fruit tissue. Background technique [0002] The plant apoplast is composed of the intracellular space outside the cell membrane, cell wall, intercellular space and differentiated mature xylem. The plant apoplast is a dynamic component, and its components are involved in important physiological and biochemical functions such as mineral nutrition and water transport, tissue shape maintenance, dynamic balance of the internal and external environment, cell growth and development regulation, signal transduction, and stress response. process. [0003] Currently, the commonly used extraction methods of plant apoplast juice are washing method and centrifugation method. In 1969, Bernstein used this method to successfully obtain leaf apoplast juice. This method rinses one side of the leaf with de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34
Inventor 张卿秦岭邢宇房克凤姜奕晨曹庆芹王建立杨柳杨瑞杨凯郝敬虹南张杰
Owner BEIJING UNIV OF AGRI
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