Method for reducing nornicotine content in leaves of burley tobacco Eyan No.3

A technology of nornicotine and burley tobacco, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve problems affecting the quality and safety of tobacco leaves

Inactive Publication Date: 2015-12-23
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The formation of the above substances seriously affects the quality and safety of tobacco leaves

Method used

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  • Method for reducing nornicotine content in leaves of burley tobacco Eyan No.3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The male parent LAB21, the female parent MSTN86 and the maintainer line TN86 of Eyan No. 3 in the group tree stage were listed and determined; two tobacco leaves at the 10th and 11th positions were taken from each tobacco plant, and were induced with 1% ethephon to promote Tobacco plants with nicotine conversion ability are transformed in advance, and the treatment time is 3 days. The main veins are removed from the treated tobacco leaves, 105 ° C for 5 minutes, and dried at 60 ° C; the content of nicotine and nornicotine in the dried samples of tobacco leaves is tested , and calculate the nicotine conversion rate of tobacco leaves; remove the male parent, female parent and maintainer tobacco strains with higher nicotine conversion rate; after continuous 2-3 generation screening, low nicotine conversion (nicotine conversion rate is less than 5% ) The nicotine conversion properties of the female parent, male parent and maintainer line of Eyan 3 are shown in Table 1. The ...

Embodiment 2

[0019] The nicotine conversion performance of Eyan No. 3 tobacco leaves before and after screening. Before screening, the nicotine conversion rate of Eyan No. 3 tobacco leaves was relatively high, and more than 40% of the tobacco plants had a nicotine conversion rate greater than 5%. Tobacco leaves with nicotine conversion greater than 5% accounted for only 6.7%.

[0020] Table 2 Nicotine conversion performance of Eyan No. 3 tobacco leaves before and after screening

[0021] Parents Nicotine conversion rate <5%

[0022] Note: There are 30 test tobacco strains in total.

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Abstract

The present invention discloses a control method of nornicotine content in leaves of burley tobacco Eyan No.3. The method is as below: continuously screening parents of burley tobacco Eyan No.3 for 2-3 generations; selecting a female parent MSTN86 with low nicotine conversion of Eyan No.3 and a maintainer line TN86 tobacco plant; and screening the current generation Eyan 3 LAB21 male parent through breeding (under permitting conditions also screening parental filter); screening a male parent and a female parent with low nicotine conversion, and conducting cross-breeding. The method effectively controls the germplasm quality of Eyan No., reduces the nornicotine content of tobacco after air-curing, and improves the safety of tobacco.

Description

technical field [0001] The invention relates to a method for reducing the nornicotine content of tobacco leaves produced by Burley tobacco Eyan No. 3 variety, and belongs to the technical field of tobacco leaf production. Background technique [0002] Common tobacco is rich in alkaloids, among which nicotine is the most abundant, usually accounting for more than 93% of the total alkaloids, and the content of nornicotine is usually less than 3.5% of the total alkaloids. However, in the cultivated Burley tobacco population, there are often individuals with higher nornicotine content and lower nicotine content, and the appearance of these individuals is due to the genetic mutation of the tobacco strain. Nornicotine is a secondary amine alkaloid, which is more unstable than nicotine, a tertiary amine alkaloid, and is prone to oxidation, acylation and nitrosation in the process of tobacco leaf preparation and aging. Generates mesmin, acylated nornicotine and NNN (N-nitrosonornic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 蔡联合杨军田兆福韦建玉孙建生邓宾玲白森胡亚杰金亚波张纪利
Owner CHINA TOBACCO GUANGXI IND
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