Compound microbial preparation and application thereof

A technology of compound microorganisms and preparations, applied in the field of silage, can solve problems such as instability and poor fermentation effect, and achieve the effect of eliminating spoilage, improving feed palatability and good fermentation effect.

Inactive Publication Date: 2015-12-23
武汉尚远环保股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the grain amaranth silage is fermented at present, most of them use a single bacterial agent, which has poor fermentation effects and selectivity problems, and often appears unstable.

Method used

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  • Compound microbial preparation and application thereof
  • Compound microbial preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Strains

[0029] Photosynthetic bacterial strains, lactic acid bacterial strains, yeast bacterial strains, actinomycete bacterial strains, and filamentous bacterial strains were purchased from China Industrial Microbiology Culture Collection and Management Center. It can also be a commercially available product, which is not limited here, and all bacteria belonging to the above-mentioned types can be used, wherein the actinomycete strain is preferably a high-temperature actinomycete strain, and the filamentous bacteria strain is preferably Aspergillus oryzae or Niger Aspergillus. The yeast bacterial strain is preferably Saccharomyces cerevisiae, brewer's yeast and the like. The lactic acid bacteria species may be lactobacillus or the like.

[0030] (2) culture medium

[0031] Photosynthetic bacteria slant medium: sodium acetate 1.145g / L, peptone 0.055g / L, sodium bicarbonate 0.6g / L, sodium thiosulfate 0.4g / L, sodium chloride 0.3g / L, magnesium sulfate 0.1g / L , Pot...

Embodiment 2

[0042] The preparation mode of photosynthetic bacteria inoculum, lactic acid bacteria inoculum, yeast bacteria inoculum, actinomycetes inoculum, and filamentous bacteria inoculum is different from Example 1 in that the inclined-plane thalline-activation (photosynthetic bacteria, lactic acid bacteria adopt corresponding Culture medium), yeast bacteria, actinomycetes, and filamentous bacteria use beef extract peptone agar medium)—according to 8% (volume percentage content) inoculum size, insert wort medium 30 ° C, 100 RPM shaker culture 30h —15% (volume percentage content) inoculum amount is connected to the reaction kettle (wort medium) at 30°C and 150RPM for 30h—the cultured photosynthetic bacteria agent, lactic acid bacteria agent, yeast bacteria agent, actinomycete bacteria The agent and the filamentous bacteria agent were inserted into the solid fermentation medium at a ratio of 0.5:1—incubate at 35°C for 30 hours—collect the bacteria and make powder, and dry it for later us...

Embodiment 3

[0046] The preparation mode of photosynthetic bacteria inoculum, lactic acid bacteria inoculum, yeast bacteria inoculum, actinomycetes inoculum, and filamentous bacteria inoculum is different from Example 1 in that the inclined-plane thalline-activation (photosynthetic bacteria, lactic acid bacteria adopt corresponding Culture medium), yeast bacteria, actinomycetes, and filamentous bacteria use beef extract peptone agar medium)—according to 15% (volume percentage) inoculum size, insert wort medium for 20 ℃, 200RPM shaker culture 20h —25% (volume percentage content) inoculum amount is connected to the reaction kettle (wort medium) at 20°C and 100RPM for 20h—the cultured photosynthetic bacteria agent, lactic acid bacteria agent, yeast bacteria agent, actinomycete bacteria Inoculate into solid fermentation medium at a ratio of 2:1 for filamentous bacterial agent and filamentous bacterial agent—incubate at 25°C for 20 hours—collect the bacterial cells to make powder, and dry for la...

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Abstract

The invention discloses a compound microbial preparation. The preparation is mainly prepared by mixing five inoculants and an auxiliary in the mass ratio being (20-30):(70-80), wherein the five inoculants comprise an inoculant of photosynthetic bacteria, an inoculant of lactic acid bacteria, an inoculant of yeast bacteria, an inoculant of actinobacteria and an inoculant of filamentous bacteria, wherein the mass ratio of the inoculant of photosynthetic bacteria to the inoculant of lactic acid bacteria to the inoculant of yeast bacteria to the inoculant of actinobacteria to the inoculant of filamentous bacteria is (2-3):(2-3):( 1-2):( 3-4):( 1-2). The invention further discloses an application of the compound microbial preparation in preparation of grain amaranth silage. The compound microbial preparation has a good fermentation effect during fermentation of the grain amaranth silage and can improve the quality of the grain amaranth silage. The invention further discloses the application of the compound microbial preparation in preparation of the grain amaranth silage.

Description

technical field [0001] The invention belongs to the technical field of silage, and in particular relates to a compound microbial preparation and its application. Background technique [0002] Grain amaranth, also known as Qiansuigu, Xiniangu, and pig amaranth, is a high-quality grain amaranth cultivated and domesticated from wild amaranth in South America. my country is also one of the places of origin, and it is an excellent feed for herbivorous livestock such as cattle, sheep, pigs, chickens, rabbits, and geese. Grain amaranth is an annual herbaceous plant. Its main features are strong adaptability, wide planting range, high yield, strong disease resistance, good palatability and strong regeneration ability. The grain amaranth is tender, juicy, fragrant and delicious, with good palatability and rich nutrition. It is a high-quality feed for livestock and poultry. It is suitable for fresh feeding, silage or preparation of high-quality grass grains. The crude protein conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/14A23K1/00
Inventor 胡小勇闫碧霄
Owner 武汉尚远环保股份有限公司
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