Cigarette essence containing lucid ganoderma fermentation broth and preparation method thereof
A technology of Ganoderma lucidum fermentation and tobacco flavor, applied in the field of cigarette flavor, can solve the problem of unclear effect of Ganoderma lucidum fermentation liquid on smoke promotion, and achieve the effects of stable product quality, clean and comfortable aftertaste, and soft and delicate taste.
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Embodiment 1
[0021] (1) Repurchase Ganoderma lucidum strain with bacterial number 5.0065 from the General Microbiology Center of China Microbiological Culture Collection Management Committee, and keep it for later use;
[0022] (2) Preparation of PDA slant solid medium: Weigh 200g of peeled potatoes, cut them into small pieces with a side length of 1-2cm, add them to 1000mL of water, boil for 30min, filter them with 4 layers of gauze, rehydrate the filtrate to 1000mL, and then add glucose 20.0 g, KH 2 PO 4 2.0g, MgSO 4 ·7H 2 O1.0g, VB trace, agar 15.0g, adjust pH to 6.0, heat and melt, pack into containers, sterilize at 121°C for 20min, and cool;
[0023] (3) Inoculate the Ganoderma lucidum strain with bacterial number 5.0065 on the PDA slant solid medium prepared in (2), culture at 28°C for 6 days, wait until the slant solid medium is covered with hyphae, and set aside;
[0024] (4) Preparation of primary fermentation medium: Add 1.00g corn flour, 1.00g bean cake flour, 0.8g bran, 1.5...
Embodiment 2
[0032] (1) Purchasing strains, preparing PDA slant solid medium, and preparing primary and secondary fermentation broths are the same as in Example 1;
[0033] (2) Inoculate the Ganoderma lucidum strain with bacterial number 5.0065 on the PDA slant solid medium prepared in (1), culture it at 26°C for 7 days, wait until the slant solid medium is covered with hyphae, and set aside;
[0034](3) Pick 4 pieces of ganoderma lucidum strains cultivated in (2) from the inclined solid medium under aseptic conditions with a side length of 3mm, put them into the primary fermentation culture medium, and then place them in constant temperature oscillation Cultivate on a shaking table, and cultivate for 5 days under the condition of the rotating speed of the shaking table at 120r / min and 28°C. When the culture liquid is clear and many small uniform mycelium balls are formed, the primary fermentation liquid of Ganoderma lucidum is made;
[0035] (4) Inoculate the primary fermentation broth cu...
Embodiment 3
[0040] (1) Purchasing bacterial strains, preparing PDA slant solid medium, and preparing primary and secondary fermentation broths are the same as in Example 1;
[0041] (2) Inoculate the Ganoderma lucidum strain with bacterial number 5.0065 on the PDA slant solid medium prepared in (1), culture it at 30°C for 5 days, wait until the slant solid medium is covered with hyphae, and set aside;
[0042] (3) Pick 5 pieces of ganoderma lucidum strains cultivated in (2) from the inclined solid medium under aseptic conditions with a side length of 5mm, put them into the primary fermentation culture medium, and then place them in constant temperature oscillation Cultivate on a shaking table, and cultivate for 7 days under the condition of the rotating speed of the shaking table at 180r / min and 26°C. When the culture liquid is clear and many uniform small mycelium balls are formed, the primary fermentation liquid of Ganoderma lucidum is made;
[0043] (4) Inoculate the primary fermentati...
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