Petiole seedling culture method for pinellia cordata
A technology of bead petiole and dripping bead, which is applied in the field of plant seedlings, can solve the problems of reduced wild area in the growth environment, low yield, and high cost of artificial planting, and achieve the effects of relieving dormancy, high emergence rate, and shortening the planting cycle
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Embodiment 2、1
[0012] Embodiment 2, 1. Take the fresh petiole of the wild dripping pearl, cut it into segments with a length of 1.5 cm, wash it with sterile water, and soak it in a D-fructose solution with a concentration of 400 ppm and a temperature of 15° C. for 28 hours; 2 1. Place the segmental petiole in running water and rinse it clean; 3. Inoculate the segmental petiole in a sterile medium and cultivate it at 18°C for 2 days. The sterile medium consists of 15 parts of agar, D-(+)-pyridine Mix 1 part of glucopyranose, 0.02 parts of 6-BA, 1-2 parts of vitamin c, and 1000 parts of distilled water, adjust the pH to 5.2, and obtain it after sterilization; 4. Rinse the segmented petiole under sterile water and place it in Carry out rooting culture in the rooting medium, the rooting culture temperature is 22 ℃, light is 20klx, the rooting medium consists of 1 part of seaweed extract, 1 part of chitosan, 10 parts of corn germ powder, 10 parts of potato starch, 20 parts of quartz powder, Pre...
Embodiment 3、1
[0014] Example 3, 1. Take the fresh petiole of the wild dripping pearl, cut it into segments with a length of 1.5 cm, wash it with sterile water, and soak it in a D-fructose solution with a concentration of 400 ppm and a temperature of 13°C for 24-2826 hours ; 2. Rinse the segmental petiole in running water; 3. Inoculate the segmental petiole in a sterile medium and cultivate it at 16°C for 2 days. The sterile medium consists of 15 parts of agar, D-(+) - After mixing 1 part of glucopyranose, 0.02 parts of 6-BA, 1-2 parts of vitamin c, and 1000 parts of distilled water, adjust the pH to 5.2 and obtain it after sterilization; 4. Rinse the segmented petiole under sterile water, Place in rooting medium for rooting culture, rooting culture temperature is 21 ℃, light is 19klx, rooting medium consists of 1 part of seaweed extract, 1 part of chitosan, 10 parts of corn germ powder, 10 parts of potato starch, 20 parts of quartz powder part, 0.06 part of ferrous sulfate, and 500 parts of...
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