Use of zoledronic acid for preparing medicine for treating fatty liver disease
A technology of fatty liver disease and zoledronic acid, which is applied in the field of zoledronic acid for the preparation of drugs for the treatment of fatty liver disease, achieving the effects of low cost development, slowing down of steatosis, and weight loss
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[0034] Method 2 Preparation of paraffin sections
[0035] Take an appropriate amount of the right lobe of liver tissue, fix it with 4% paraformaldehyde for 24-48h, dehydrate with ethanol step by step, transparent xylene, immerse in wax, embed in paraffin, and section with a thickness of 5μm.
[0036] Method 3 HE staining of paraffin sections
[0037] The sections were immersed in xylene for 10 min and 5 min to dewax, and then water was added to gradient ethanol. After dyeing with hematoxylin for 4 minutes, wash with distilled water. Use 0.25% NH 3 ·H 2 O pan-blue 30s and then rinse with distilled water. After dehydration with gradient concentration of ethanol to 95% ethanol, 0.5% eosin solution is stained for 2-3 seconds, then dehydrated in 100% ethanol, and soaked in xylene for 2 minutes. Finally, seal the sheet with neutral gum.
[0038] Method 4 Preparation of frozen sections
[0039] An appropriate amount of tissue was taken from the same part of the right lobe of the experiment...
Embodiment 1
[0052] Example 1 The effect of zoledronic acid on food-borne non-alcoholic fatty liver
[0053] Clinically, patients have fatty liver, and their liver is swollen in appearance, but the main reason is the accumulation of lipids. The accumulation of this lipid is obtained by intravital puncture to obtain liver tissue, and the accumulation of lipids can be seen by HE staining and oil red staining. Therefore, direct detection of HE and oil red is a better way to observe lipid accumulation.
[0054] According to the aforementioned general materials and methods, zoledronic acid and placebo were first administered to mice in the high-fat diet group. In this embodiment, zoledronic acid is mainly administered by tail vein injection, and the main site of action of the drug is the liver, which is injected once every two days. The dosage is 50μg / kg zoledronic acid. Tissue triglyceride level detection and HE staining were performed on the mice after administration. The experimental data are s...
Embodiment 2
[0061] Example 2 The effect of zoledronic acid on food-borne fatty liver damage
[0062] Similar to Example 1, according to the aforementioned general materials and methods, firstly, zoledronic acid and placebo were used to administer high-fat-induced mice, and the alanine aminotransferase (ALT) and aspartate aminotransferase (AST ) Level, the experimental data is shown in Table 3, and the results are as Figure 4 Shown in A and 4B.
[0063] Table 3 The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the blood of mice after administration
[0064]
[0065]
[0066] The HFD group was a high-fat diet group, the RC group was a normal diet group, PBS was a placebo, ZOL was zoledronic acid, and the dose was 50μg / kg.
[0067] It can be seen from Table 3 that for the HFD group, after ZOL administration, the levels of ALT and AST were significantly reduced. Among them, in the RC group, ZOL administration compared with PBS administration, ALT and AST did not c...
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