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Application of oroxin B and drug containing oroxin B

A technology of oreoside and medicine, applied in the field of medicinal chemistry

Active Publication Date: 2016-01-13
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no reports on the effect of oreoside B on this pathway

Method used

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  • Application of oroxin B and drug containing oroxin B
  • Application of oroxin B and drug containing oroxin B
  • Application of oroxin B and drug containing oroxin B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Inhibitory effect of orinetoside B on the growth of various tumor cells

[0046] Experimental Materials:

[0047] (1) Tumor cell lines

[0048] The 30 strains of human tumor cells used in the anti-tumor cell test of oreoside B are as follows: human Burkitt's lymphoma cells (Raji, Namalva), human T-lymphocytic leukemia cells (Jurkat), human melanoma highly metastatic cell lines (C8161), Human monocytic leukemia cells (THP-1), human myeloid leukemia cells (HL-60), human T lymphocytes (H9), human histiocytic lymphoma cells (U937), human glioma cells (SHG44) , human chronic myelogenous leukemia cells (K562), human brain astroglioblastoma (U-87MG), human gastric cancer cells (HGC-27), human cecal adenocarcinoma cells (Hce-8693), human glioma Cell tumor cells (A172), human malignant embryonic rhabdomyoma cells (RD), human osteosarcoma cells (U-2OS, LP-1), human liver cancer cells (HepG2, HCCC-9810), human cervical cancer intestinal metastasis cells (Caski) , huma...

Embodiment 2

[0059] Example 2 Selective Inhibitory Effect of Oriperoside B on the Growth of Malignant Lymphoma Cells in Vitro

[0060] Experimental Materials:

[0061] (1) Tumor cell lines: B lymphoma Raji and Namalwa cell lines, and non-tumorigenic lymphoblastoid HMY2-CIR were derived from ATCC in the United States. The cell culture medium DMEM (high sugar) is a product of Hyclone Company.

[0062] (2) Tested drug: Oreoside B was solubilized with dimethyl sulfoxide (DMSO), and diluted with DMEM (high glucose) medium to the desired concentration (0-40 μmol / L).

[0063] experimental method:

[0064] Lympholyte-H is used to separate the blood of normal people. The main steps of the experiment are: After drawing the peripheral blood of normal people (adding heparin), the peripheral blood is slowly added to the centrifuge tube with Lympholyte-H separation solution In the process, centrifuge to remove the middle mononuclear cell layer, wash twice with PBS, remove the separation fluid and pla...

Embodiment 3

[0069] Example 3 Oriperoside B effectively reduces the tumorigenicity of malignant lymphoma cells

[0070] Experimental Materials:

[0071] (1) Tumor cell line: B lymphoma Raji was derived from ATCC in the United States. The cell culture medium DMEM (high sugar) is a product of Hyclone Company.

[0072] (2) Tested drug: Oreoside B was solubilized with dimethyl sulfoxide (DMSO), and diluted with DMEM (high glucose) medium to the desired concentration (0-20 μmol / L).

[0073] experimental method:

[0074] Colony formation ability is the gold standard for measuring cell tumorigenicity, so this embodiment uses colony formation experiments to determine whether the tumorigenicity of malignant lymphoma Raji cells after being treated with oreoside B is reduced. The specific method is: Raji cells are treated with different After 48 hours of treatment with oleoside B (0, 5, 10, 20 μmol / L), the cells were collected and counted, and 600 viable cells were taken for each concentration and...

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Abstract

The invention belongs to the technical field of medicinal chemistry, and discloses the application of the oroxin B and a drug containing the oroxin B. The invention further provides the application of the oroxin B in preparing a tumor prevention and treatment drug, an MKK3 agonist, a P38 agonist and a DDIT3 accelerant. The oroxin B has an excellent anti-cancer effect in vivo and in vitro, particularly has a relatively stronger inhibiting effect on malignant lymphoma, and has no obvious toxic and side effects. The oroxin B has an antitumor mechanism that the oroxin B can induce lymphoma cells to be in disastrous vacuolus and oncosis, and can effectively weaken the oncogenicity of tumour cells; the oroxin B has a molecular mechanism that by activating the MKK3-P38 access of a stress reaction system in the lymphoma cells, the expression of a key tumor suppressor gene DDIT3 is promoted, and endoplasmic reticulum stress and tumour cell oncosis in cells can be activated specifically, therefore, an anti-cancer effect is played; and no obvious restraining effect can be played on normal lymphocyte, and further leukemia and blood platelet in body's blood can be promoted moderately.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry, and in particular relates to the application of oreoside B and medicines containing oreoside B. Background technique [0002] Wood butterfly is a commonly used traditional Chinese medicine. "Chinese Pharmacopoeia" records its source as the dry mature seeds of Bignoniaceae plant wood butterfly, also known as Qianzhangzhi. Wood Butterfly is bitter, sweet, and cool in nature and taste, enters the lung, liver, and stomach meridians, and has the effects of clearing the lung, relieving the throat, relieving cough, soothing the liver and harmonizing the stomach, and promoting muscle growth. Modern medical research has shown that wood butterfly has various pharmacological effects such as analgesia, anti-inflammation, antibacterial, anti-oxidation, inhibition of virus and tumor growth, but it is still unclear which component plays the main role. Studies have found that the chemical compositio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00A61P35/02
Inventor 周泉生曹志飞傅士龙
Owner SUZHOU UNIV
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