Application of nano-selenium as radiosensitizer of iodine‑125 particles

A technology of nano selenium and sensitizer, applied in the field of tumor treatment

Active Publication Date: 2018-03-27
陈填烽 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, targeted therapy based on the differences between tumor cells and normal cells is currently a direction with a lot of research but very limited progress.

Method used

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  • Application of nano-selenium as radiosensitizer of iodine‑125 particles
  • Application of nano-selenium as radiosensitizer of iodine‑125 particles
  • Application of nano-selenium as radiosensitizer of iodine‑125 particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Preparation and characterization of embodiment 1 nano-selenium

[0052] In this embodiment, three kinds of nano-selenium were designed and synthesized, which are polyethylene glycol (PEG) dispersed nano-selenium (PEG-SeNPs) prepared by physically dissolving elemental gray selenium, and vitamin C (VC) was used to reduce selenous acid (H 2 SeO 3 ) or sodium selenite (Na 2 SeO 3 ), and then use PEG or polyvinylpyrrolidone (PVP) to modify nano-selenium to obtain (PEGW-SeNPs, PVP-SeNPs), and detect their responsiveness to radiation, select the optimal material for further activity screening, and Study its co-action mechanism with X-rays. The following preparation methods of nano-selenium are currently known and commonly used conventional methods, and are not intended to limit the present invention.

[0053] (1) Preparation of PEG-SeNPs: Reference [Zheng SY, Li XL, Zhang YB, Xie Q, Wong YS, ZhengWJ, et al. PEG-nanolized ultrasmall selenium nanoparticles overcome drug resi...

Embodiment 2

[0061] Example 2 Study on anti-tumor activity of PEG-SeNPs and radioactive particles I-125 in vitro against cervical cancer

[0062] This example uses PEG-SeNPs. Based on the electron-ray sensitivity characteristics of SeNPs, we look forward to developing applications of SeNPs in the field of radiotherapy. However, ordinary radiotherapy has its limitations and deficiencies. For example, even if the lesion is not completely controlled or new metastases appear after reaching the maximum dose, it is still not suitable to undergo radiotherapy again in a short period of time. Some tumors grow near important organs or radiation-sensitive tissues, which also limits the use of radiotherapy. Thus, radioactive seed implantation brachytherapy was born as a new type of radiotherapy. In this experiment, we studied the in vitro antitumor activity of functionalized nano-selenium (SeNPs) combined with radioactive particles I-I-125 and radiotherapy. After HeLa cells were cultured for 24 hou...

Embodiment 3

[0068] Example 3 In vitro anti-breast cancer tumor activity of functionalized nano-selenium FA@CS-SeNPs and radioactive particles I-I-125

[0069] Preparation of nano-selenium: refer to the following literature

[0070] Bo Yu, Xiaoling Li, Wenjie Zheng, *Yanxian Feng, Yum-Shing Wong and Tianfeng Chen*.pH-Responsive Cancer-targeted Selenium Nanoparticles: A Transformable Drug Carrier with Enhanced Theranostic Effects, J.Mater.Chem.B, 2014, 2( 33), 5409-5418. The reaction steps are as follows:

[0071] (1) Preparation of CS-coupled folic acid (CS-FA): Weigh an appropriate amount of FA and dissolve it in DMSO (FA final concentration is 10-50 mg / ml), add appropriate amount of NHS and EDC (FA:NHS:EDC= 1: 0.5-2: 0.5-2) stirring and reacting for 2-24 hours to activate folic acid, then adding chitosan solution (final concentration is 0.1-10 mg / ml) and mixing evenly, stirring and reacting for 12-48 hours, dialyzed for 12 hours -48 hours, FA@CS solution was obtained.

[0072] (2) H ...

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Abstract

The invention discloses an application of nano-selenium as an iodine-125 particle radiotherapy sensitizing agent. PEG is used for dissolving simple substance selenium for preparing nano-selenium. The nano-selenium also comprises nano-selenium modified by polysaccharide and chitosan as well as tumor targeting nano-selenium (SeNPs) modified by folic acid and transferrin. SeNPs has a stimuli-response characteristic to iodine-125, and a synergism is formed by the SeNPs and gamma-ray produced by iodine-125; the SeNPs has a high-efficiency inhibition effect for MCF-7 breast cancer cells according to in vitro cell experiments and mouse in vivo experiments, and can obviously inhibit propagation of human cervical carcinoma cell Hela, melanoma cell A375, breast cancer cell MCF-7 and glioma cell C6 and a plurality of cells. The mechanism is mainly as follows: accumulation of active oxygen in the cells is enhanced, which induces cell cycle arrest and tumor cell apoptosis. The result prompts that SeNPs can be used as a novel radiotherapy sensitizing agent for development.

Description

technical field [0001] The invention relates to the technical field of tumor treatment, in particular to the application of nano-selenium as a radiosensitizer for iodine-125 particles. Background technique [0002] In the past few decades, radiotherapy has developed rapidly as an important means of clinical treatment of tumors. Studies have shown that about 50% of tumor patients have received radiotherapy alone or combined with other means. Among them, iodine-125 seed implantation radiotherapy is an emerging radiotherapy method, and its curative effect has been widely recognized. Iodine-125 radioactive particles, also known as particle knife, refer to the three-dimensional stereotactic radiotherapy system between (minimally invasive) tissues in cancer treatment. Tumor treatment methods. Its main feature is that it can permanently embed iodine-125 radioactive particles into the tumor body, sub-tumor area and possible metastatic lymphatic pathway for continuous treatment, or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/60A61K47/61A61K47/64A61K9/14A61P35/00A61K51/00A61K101/02
Inventor 陈填烽谢强
Owner 陈填烽
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