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A kind of lipase detection kit

A technology for detecting kits and lipase, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve problems such as waste, poor accuracy, and the impact of reagent stability , to achieve the effect of enhancing stability, increasing stabilization time, and excellent stability performance

Active Publication Date: 2018-05-22
郁东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of enzymes in the ordinary lipase enzyme color detection reagent, the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in poor accuracy and waste.

Method used

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  • A kind of lipase detection kit
  • A kind of lipase detection kit
  • A kind of lipase detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A lipase kit with excellent accuracy recognized on the market, which includes reagent R1, reagent R2, and calibrator.

[0034] Wherein the reagent R1 consists of:

[0035] pH 7.2 Tris buffer 100 mmol / L

[0036] Sodium chloride 40 mmol / L

[0037] Taurodeoxycholic acid 35 mmol / L

[0038] Reagent R2 consists of:

[0039] Tartrate buffer pH 4.0 9.5mmol / L

[0040] Co-lipase 500U / L

[0041] 1,2 o-Dilaurinyl glycerol-3-glutaric acid-(6’-methylresorufin)-ester 0.2 mmol / L

[0042] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0043] Add 3 μl of physiological saline, sample or calibrator, then add 200 μl of R1 reagent for pre-incubation for 5 minutes, then add 100 μl of reagent R2 and react for 5 minutes, read the absorbance value twice, and calculate ΔA / min.

[0044] The calibrator used in this embodiment is a composite calibrator produced by Landa...

Embodiment 2

[0046] A lipase detection kit, which includes reagent R1, reagent R2, and calibrator.

[0047] Wherein the reagent R1 consists of:

[0048] pH 7.2 Tris buffer 100 mmol / L

[0049] Sodium chloride 40 mmol / L

[0050] Taurodeoxycholic acid 35 mmol / L

[0051] Reagent R2 consists of:

[0052] Tartrate buffer pH 4.0 9.5mmol / L

[0053] Co-lipase 500U / L

[0054] 1,2-o-dilaurinylglycerol-3-pentanedi

[0055] Acid-(6'-methylresorufin)-ester 0.2 mmol / L

[0056] 4-FPBA 0.01%

[0057] Glucomannan 0.5 mg / mL

[0058] NaCl 5mmol / L

[0059] Propylene glycol 20mg / mL

[0060] Triton 100 1%

[0061] BSA 0.1g / L

[0062] Concrete determination method is with embodiment 1.

Embodiment 3

[0064] A lipase detection kit, which includes reagent R1, reagent R2, and calibrator.

[0065] Wherein the reagent R1 consists of:

[0066] pH 7.2 Tris buffer 100 mmol / L

[0067] Sodium chloride 40 mmol / L

[0068] Taurodeoxycholic acid 35 mmol / L

[0069] Reagent R2 consists of:

[0070] Tartrate buffer pH 4.0 9.5mmol / L

[0071] Co-lipase 500U / L

[0072] 1,2-o-dilaurinylglycerol-3-pentanedi

[0073] Acid-(6'-methylresorufin)-ester 0.2 mmol / L

[0074] 4-FPBA 0.01%

[0075] Glucomannan 0.8 mg / mL

[0076] NaCl 5mmol / L

[0077] Propylene glycol 20mg / mL

[0078] Triton 100 1%

[0079] BSA 0.5g / L

[0080] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses a lipase detection kit, which belongs to the technical field of clinical in vitro detection reagents. The kit of the present invention includes reagent R1, reagent R2, and calibrator. By adding a composite stabilizer consisting of 4-FPBA, glucomannan, NaCl, propylene glycol, Triton 100, and BSA to reagent R2, the stability of the kit is effectively improved, and its linear range is also good. The accuracy is high, which is conducive to further promotion and use in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a lipase detection kit. Background technique [0002] Lipase (Lipase, glyceride hydrolase, LPS) belongs to carboxyl ester hydrolase, is an enzyme that hydrolyzes long-chain fatty acid glycerides, and can gradually hydrolyze triglycerides into glycerol and fatty acids. one of the enzymes. In addition to serum, lipase activity can also be detected in stomach, small intestinal mucosa, lung, white blood cells, fat cells, milk, etc. In normal blood, there is only a small amount of lipase, which is easily cleared by the kidneys. When the secretion of the pancreas is hypersecreted, the pancreatic duct is blocked, or the pancreas is damaged or necrotic, the lipase flows backward or directly into the blood, making the lipase in the blood active. Increase. Under normal circumstances, the concentration of LPS in the pancreas is 20,000 times that of the serum,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 郁东董雯甘宜梧谢清华谭柏清
Owner 郁东
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