Haptoglobin detection kit with high sensitivity
A technology of haptoglobin and detection kits, which is applied in the field of haptoglobin detection kits, can solve the problems of low accuracy, poor stability, complicated operation, etc., and achieve the effect of excellent stability
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Embodiment 1
[0026] A highly accurate haptoglobin kit approved on the market, including reagent R1, reagent R2, and calibrator.
[0027] Wherein the reagent R1 consists of:
[0028] Phosphate buffer (pH7.4) 20mmol / L
[0029] Polyethylene glycol (PEG) 1g / L
[0030] Reagent R2 consists of:
[0031] Goat anti-human HP antibody >10mL / L
[0033] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:
[0034] Add 3 μl of purified water, sample or calibrator, then add 240 μl of R1 reagent for pre-incubation for 5 minutes, then add 60 μl of reagent R2, start recording the absorbance value A1 after 30 seconds, and read the absorbance A2 after 5 minutes of reaction, and calculate ΔA / min.
[0035] The calibrator used in this embodiment is a composite calibrator produced by Landau, UK.
Embodiment 2
[0037] A haptoglobin detection kit with high sensitivity comprises reagent R1, reagent R2 and calibrator.
[0038] Wherein the reagent R1 consists of:
[0039]
[0040] Reagent R2 consists of:
[0041]
[0042] Concrete determination method is with embodiment 1.
Embodiment 3
[0044] A haptoglobin detection kit with high sensitivity comprises reagent R1, reagent R2 and calibrator.
[0045] Wherein the reagent R1 consists of:
[0046]
[0047] Reagent R2 consists of:
[0048]
[0049] Concrete determination method is with embodiment 1.
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