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A technology of haptoglobin and detection kits, which is applied in the field of haptoglobin detection kits, can solve the problems of low accuracy, poor stability, complicated operation, etc., and achieve the effect of excellent stability
Inactive Publication Date: 2019-01-08
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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Problems solved by technology
However, the haptoglobin detection reagents currently on the market have defects such as complicated operation, low accuracy, and poor stability, so they need to be improved.
Method used
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Embodiment 1
[0026] A highly accurate haptoglobin kit approved on the market, including reagent R1, reagent R2, and calibrator.
[0027] Wherein the reagent R1 consists of:
[0028] Phosphate buffer (pH7.4) 20mmol / L
[0029] Polyethylene glycol (PEG) 1g / L
[0030] Reagent R2 consists of:
[0031] Goat anti-human HP antibody >10mL / L
[0032] Sodium azide 1g / L
[0033] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:
[0034] Add 3 μl of purified water, sample or calibrator, then add 240 μl of R1 reagent for pre-incubation for 5 minutes, then add 60 μl of reagent R2, start recording the absorbance value A1 after 30 seconds, and read the absorbance A2 after 5 minutes of reaction, and calculate ΔA / min.
[0035] The calibrator used in this embodiment is a composite calibrator produced by Landau, UK.
Embodiment 2
[0037] A haptoglobin detection kit with high sensitivity comprises reagent R1, reagent R2 and calibrator.
[0038] Wherein the reagent R1 consists of:
[0039]
[0040] Reagent R2 consists of:
[0041]
[0042] Concrete determination method is with embodiment 1.
Embodiment 3
[0044] A haptoglobin detection kit with high sensitivity comprises reagent R1, reagent R2 and calibrator.
[0045] Wherein the reagent R1 consists of:
[0046]
[0047] Reagent R2 consists of:
[0048]
[0049] Concrete determination method is with embodiment 1.
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Abstract
The invention relates to the technical field of serum haptoglobin detection, in particular to a serum haptoglobin detection reagent. A reagent R1 comprises a phosphate buffer solution, polyethylene glycol, Twain-80 and a preservative; and a reagent R2 comprises the phosphate buffer soluition, Twain-80, a goat anti human HP antibody and the preservative. The reagent R1 adopts a compound stabilizerconsisting of the phosphate buffer solution and sodium azide, and the problem that long-term storage of the reagent is unstable is solved; and a compound reagent consisting of an accelerator polyethylene glycol and a surface active agent Twain-80 is added, so that the sensitivity of reagent detection is improved. Moreover, the stability of antibody protein can be improved effectively by adding thecompound stabilizer into the reagent R2; and the surface active agent Twain-80 is added, so that the sensitivity of reagent detection is improved.
Description
technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a highly sensitive haptoglobin detection kit. Background technique [0002] Haptoglobin, also known as haptoglobin, is an acidic glycoprotein with a molecular weight of 85,000 in the serum α2 globulin fraction. In the serum and other body fluids of humans and many mammals, it is mainly synthesized and degraded in the liver. [0003] The main function of haptoglobin is to bind free hemoglobin to form a stable haptoglobin-hemoglobin complex. This combination changes the configuration and characteristics of haptoglobin and hemoglobin, and this reaction is basically irreversible. . There are many clinical causes that can cause damage to red blood cells, such as trauma, burns, etc., resulting in a significant increase in free hemoglobin in the blood. Hemoglobin can be filtered from the human kidney, causing kidney damage, and even irreversible renal func...
Claims
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