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A kit for detecting high-density lipoprotein cholesterol

A high-density lipoprotein and detection kit technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as waste, poor accuracy, and reagent stability effects, and achieve enhanced stability and increased stability time , excellent effect of stability

Active Publication Date: 2020-04-28
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of enzymes in ordinary high-density lipoprotein cholesterol detection reagents, the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in poor accuracy and waste.

Method used

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  • A kit for detecting high-density lipoprotein cholesterol
  • A kit for detecting high-density lipoprotein cholesterol
  • A kit for detecting high-density lipoprotein cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A high-density lipoprotein cholesterol kit with excellent accuracy recognized in the market, which includes reagent R1, reagent R2, and calibrator.

[0043] Wherein the reagent R1 consists of:

[0044] Tris buffer (pH=7.0) 100 mmol / L

[0045] 4-aminoantipyrine 500μmol / L

[0046] Triton X-100 0.5% (V / V)

[0047] Sodium azide 0.5% (W / V)

[0048] N-Ethyl-N-(2-hydroxy-3-sulfopropyl) m-toluidine 1 mmol / L

[0049] Sodium Glucose Sulfate 0.5 g / L

[0050] Sulfurized cyclodextrin 0.1 g / L

[0051] Reagent R2 consists of:

[0052] Tris buffer (pH=7.0) 100 mmol / L

[0053] Cholesterol oxidase 500U / L

[0054] Cholesterol esterase 800U / L

[0055] Peroxidase 30KU / L.

[0056] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0057] Add 3 μl of physiological saline, sample or calibrator, then add 225 μl of R1 reagent for pre-incubation for 5 minutes and re...

Embodiment 2

[0060] A test kit for high-density lipoprotein cholesterol, which includes reagent R1, reagent R2, and calibrator.

[0061] Wherein the reagent R1 consists of:

[0062] Tris buffer (pH=7.0) 100 mmol / L

[0063] 4-aminoantipyrine 500μmol / L

[0064] Triton X-100 0.5% (V / V)

[0065] Sodium azide 0.5% (W / V)

[0066] N-Ethyl-N-(2-hydroxy-3-sulfopropyl) m-toluidine 1 mmol / L

[0067]Sodium Glucose Sulfate 0.5 g / L

[0068] Sulfurized cyclodextrin 0.1 g / L

[0069] Reagent R2 consists of:

[0070] Tris buffer (pH=7.0) 100 mmol / L

[0071] Cholesterol oxidase 500U / L

[0072] Cholesterol esterase 800U / L

[0073] Peroxidase 30KU / L

[0074] 4-FPBA 0.01% (W / V)

[0075] Glucomannan 0.5 mg / mL

[0076] NaCl 5mmol / L

[0077] Propylene glycol 20mg / mL

[0078] Triton 100 1% (V / V)

[0079] BSA 0.1g / L.

[0080] Concrete determination method is with embodiment 1.

Embodiment 3

[0082] A test kit for high-density lipoprotein cholesterol, which includes reagent R1, reagent R2, and calibrator.

[0083] Wherein the reagent R1 consists of:

[0084] Tris buffer (pH=7.0) 100 mmol / L

[0085] 4-aminoantipyrine 500μmol / L

[0086] Triton X-100 0.5% (V / V)

[0087] Sodium azide 0.5% (W / V)

[0088] N-Ethyl-N-(2-hydroxy-3-sulfopropyl) m-toluidine 1 mmol / L

[0089] Sodium Glucose Sulfate 0.5 g / L

[0090] Sulfurized cyclodextrin 0.1 g / L

[0091] Reagent R2 consists of:

[0092] Tris buffer (pH=7.0) 100 mmol / L

[0093] Cholesterol oxidase 500U / L

[0094] Cholesterol esterase 800U / L

[0095] Peroxidase 30KU / L

[0096] 4-FPBA 0.01% (W / V)

[0097] Glucomannan 0.8 mg / mL

[0098] NaCl 5mmol / L

[0099] Propylene glycol 20mg / mL

[0100] Triton 100 1% (V / V)

[0101] BSA 0.5g / L.

[0102] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses a high-density lipoprotein cholesterol detection kit, and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibration product. By adding a composite stabilizer consisting of 4-FPBA, glucomannan, NaCl, propanediol, triton 100 and BSA into the reagent R2, the stability of the kit is effectively improved; and the linear range of the kit is also relatively wide, and the reagent accuracy is high, so that the kit can be further popularized and used in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a high-density lipoprotein cholesterol detection kit. Background technique [0002] High-density lipoprotein cholesterol (HDL-cholesterol; HDL-Ch) refers to the cholesterol carried by high-density lipoprotein molecules. It is an endogenous cholesterol ester that is reverse-transported, transported into the liver, and then cleared out of the blood. High-density lipoproteins contain 20% to 30% of the total cholesterol in humans. High-density lipoprotein cholesterol can be commonly understood as "good" cholesterol, anti-atherosclerotic cholesterol, because HDL-C can reduce the risk of coronary heart disease. [0003] HDL cholesterol is composed of apolipoproteins, phospholipids, cholesterol and a small amount of fatty acids. [0004] The reference range of high-density lipoprotein cholesterol in human serum or plasma is 0.77-2.25 mmol / L. Increase: The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/92
CPCG01N33/92G01N2800/323
Inventor 董雯赵民王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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