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Glucose detection kit

A technology for detecting kits and glucose, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc. It can solve problems such as waste, poor accuracy, and influence on reagent stability, and achieve enhanced stability, increased stability time, Excellent effect of stability

Active Publication Date: 2016-03-16
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of enzymes in ordinary glucose detection reagents, the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in poor accuracy and waste.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A glucose kit with excellent accuracy recognized on the market, which includes reagent R1, reagent R2, and calibrator.

[0042] Wherein the reagent R1 consists of:

[0043] Disodium hydrogen phosphate 25mmol / L

[0044] Potassium dihydrogen phosphate 50mmol / L

[0045] Potassium ferrocyanide 0.05mmol / L

[0046] Sodium p-hydroxybenzoate 15mmol / L

[0047] 4-aminoantipyrine 500μmol / L

[0048] Peroxidase 2000U / L

[0049] TritonX-1000.5% (V / V)

[0050] Sodium azide 0.5% (W / V)

[0051] Reagent R2 consists of:

[0052] Disodium hydrogen phosphate 25mmol / L

[0053] Potassium dihydrogen phosphate 50mmol / L

[0054] Glucose oxidase 24000U / L

[0055] Disodium EDTA 10mmol / L

[0056] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0057] Add 5 μl of physiological saline, sample or calibrator, then add 400 μl of R1 reagent for pre-incubation for 5 minut...

Embodiment 2

[0060] A glucose detection kit, which includes reagent R1, reagent R2, and calibrator.

[0061] Wherein the reagent R1 consists of:

[0062] Disodium hydrogen phosphate 25mmol / L

[0063] Potassium dihydrogen phosphate 50mmol / L

[0064] Potassium ferrocyanide 0.05mmol / L

[0065] Sodium p-hydroxybenzoate 15mmol / L

[0066] 4-aminoantipyrine 500μmol / L

[0067] Peroxidase 2000U / L

[0068] TritonX-1000.5% (V / V)

[0069] Sodium azide 0.5% (W / V)

[0070] Reagent R2 consists of:

[0071] Disodium hydrogen phosphate 25mmol / L

[0072] Potassium dihydrogen phosphate 50mmol / L

[0073] Glucose oxidase 24000U / L

[0074] Disodium EDTA 10mmol / L

[0075] 4-FPBA0.01%

[0076] Glucomannan 0.5mg / mL

[0077] NaCl5mmol / L

[0078] Propylene glycol 20mg / mL

[0079] Triton 1001%

[0080] BSA0.1g / L

[0081] Concrete determination method is with embodiment 1.

Embodiment 3

[0083] A glucose detection kit, which includes reagent R1, reagent R2, and calibrator.

[0084] Wherein the reagent R1 consists of:

[0085] Disodium hydrogen phosphate 25mmol / L

[0086] Potassium dihydrogen phosphate 50mmol / L

[0087] Potassium ferrocyanide 0.05mmol / L

[0088] Sodium p-hydroxybenzoate 15mmol / L

[0089] 4-aminoantipyrine 500μmol / L

[0090] Peroxidase 2000U / L

[0091] TritonX-1000.5% (V / V)

[0092] Sodium azide 0.5% (W / V)

[0093] Reagent R2 consists of:

[0094] Disodium hydrogen phosphate 25mmol / L

[0095] Potassium dihydrogen phosphate 50mmol / L

[0096] Glucose oxidase 24000U / L

[0097] Disodium EDTA 10mmol / L

[0098] 4-FPBA0.01%

[0099] Glucomannan 0.8mg / mL

[0100] NaCl5mmol / L

[0101] Propylene glycol 20mg / mL

[0102] Triton 1001%

[0103] BSA0.5g / L

[0104] Concrete determination method is with embodiment 1.

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PUM

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Abstract

The invention discloses a glucose detection kit and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibrator. A composite stabilizer prepared from 4-FPBA, glucomannan, NaCl, propylene glycol, triton100 and BSA is added into the reagent R2, so that the stability of the kit is effectively improved, the linear range is better, the reagent accuracy degree is high, and the glucose detection kit is beneficial to being used and popularized further in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a glucose detection kit. Background technique [0002] Glucose (Glucose) (chemical formula C6H12O6) is also known as corn glucose, corn sugar, referred to as glucose. English aliases Dextrose, Cornsugar, Grapesugar, Bloodsugar. It is the most widely distributed and most important monosaccharide in nature, and it is a polyhydroxy aldehyde. Pure glucose is a colorless crystal with a sweet taste but not as sweet as sucrose (ordinary people cannot taste the sweet taste), easily soluble in water, slightly soluble in ethanol, and insoluble in ether. The optical rotation of the aqueous solution is to the right, so it belongs to "dextrose". Glucose plays an important role in the field of biology. It is the energy source and metabolic intermediate product of living cells, that is, the main energy supply material for organisms. Plants can produce glucose th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/54C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/54
Inventor 李静董雯谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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