Artificial breeding method for glyptosternon maculatum
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It is a technology of artificial breeding and application in fish farming, application, climate change adaptation, etc. It can solve problems such as non-compliance with energy saving and emission reduction, and achieve the effects of high quality and safety, high density and high resource utilization.
Inactive Publication Date: 2016-01-20
TIBET ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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In June 2008, the artificial breeding of P. black spot was successful, but no substantive progress has been made in the artificial domestication and large-scale breeding. Before the intensive cultivation of P. The biological bait in the original facility is turned to artificial compound feed, which is convenient for artificial large-scale breeding
The patented method adopts artificial cultivation in cement ponds, which does not meet the needs of modern artificial breeding for energy saving and emission reduction
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Embodiment 1
[0047] A kind of artificial breeding method of black-spotted catfish , including the following steps:
[0048] A. Selection of broodstock
[0049] After soaking in salt water, the wild black-spotted broodstock are identified as males and females, and the females and males are collected;
[0050] B. Artificial induced labor
[0051] The dosage of the pharmaceutical composition for inducing labor in female fish is: luteinizing hormone releasing hormone 5 μg / kg body weight + 1 carp pituitary gland / kg body weight; 24 hours after injection, lightly press the abdomen of female fish, if no free eggs flow out, then inject The second injection of the same dose of oxytocin;
[0052] C. Artificial insemination
[0053] Fertilization by dry method: take out the testis of the male fish, grind it, wash it with normal saline, put it into a container containing fish eggs, mix the sperm and eggs, put them into the hatching tray, and hatch them in the hatching tank;
[0054] D. seedling cu...
Embodiment 2
[0057] This embodiment is on the basis of embodiment 1:
[0058] In step A, soak for 20 minutes in saline with a mass fraction of 1%.
Embodiment 3
[0060] This embodiment is on the basis of embodiment 1:
[0061] In the step A, soak for 40 minutes in saline with a mass fraction of 1%.
[0062] Described A step, the body weight of collecting female fish is 0.065kg, and the body weight of male fish is 0.38kg.
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Abstract
The invention provides an artificial breeding method for glyptosternon maculatum. The artificial breeding method includes the following steps that A, after wild glyptosternon maculatum parent fishes are immersed in a saline solution, sex discrimination is conducted, and female fishes and male fishes are collected; B, a medicine composition for female fish spawning induction is prepared from 5 micrograms of lutropin releasing hormones and one carp hypophysis per kg of the female fishes, 24 hours after injection, the abdomens of the female fishes are slightly pressed, and if no free egg granule flows out, a second shot of spawning induction medicine of the same dosage is injected; C, dry method fertilization is adopted; D, after egg membranes of zygotes disengage, the zygotes are poured into an incubation tank to be incubated, when glyptosternon maculatum fry begin to swim at the bottom of a pool, exogenous nutrition is turned to, and animal live bait and artificial compound feed are fed. The nature rule of the wild glyptosternon maculatum parent fishes is grasped, the artificial propagation technology of glyptosternon maculatum is studied, a set of feasible artificial spawning induction and artificial incubation method is formed, and artificial large-scale breeding of glyptosternon maculatum is achieved.
Description
technical field [0001] The invention relates to the aquaculture industry, in particular to an artificial breeding method of black-spotted catfish. Background technique [0002] Siluriformes Siluriformes , Anguidae Sisoridae Prototheca Glyptosternum . It is a plateau cold-water fish, distributed in the Brahmaputra River in India and Linzhi, Tangjia, Shigatse and other areas in my country's Tibet. The distribution altitude ranges from about 4200m to about 2800m. It is lower than 15°C all year round. Black-spotted catfish as a catfish ( Glyptosternoids fish), its early studies were mostly in taxonomy, biogeography and evolution; in recent years, most studies have focused on the phylogeny and evolution of catfish. Black spotted catfish is an omnivorous fish with a partial feeding habit. It eats more than 40 kinds of bait, and fish is its main bait, followed by algae, and thirdly oligochaetes and organic debris. Black spotted catfish is mainly distributed in the middle a...
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