Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of method that acidic amino acid hydrolyzes ginsenoside to prepare ginseng rare saponin

A technology of rare ginsenosides and acidic amino acids, applied in organic chemistry, steroids, etc., can solve the problems of strong corrosiveness, poor hydrolysis specificity of ginsenosides, and low yield, etc. one-off effect

Active Publication Date: 2017-10-31
JILIN YATAI PHARM CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention discloses a method for preparing rare ginsenosides by using acidic amino acids to catalyze the hydrolysis of ginsenosides, which can efficiently and environmentally friendly hydrolyze ginsenosides to prepare rare ginsenosides, and solve the problem of poor hydrolysis specificity of ginsenosides caused by the use of a large amount of strong acid and strong alkali in the existing preparation process , low yield, strong corrosiveness, environmental pollution and many by-products, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method that acidic amino acid hydrolyzes ginsenoside to prepare ginseng rare saponin
  • A kind of method that acidic amino acid hydrolyzes ginsenoside to prepare ginseng rare saponin
  • A kind of method that acidic amino acid hydrolyzes ginsenoside to prepare ginseng rare saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020]Panaxadiol group saponins or triol group saponins are dissolved in water according to the mass volume ratio of 1:1 (kg / liter), and acidic amino acids are added, so that the mass ratio of panaxadiol group saponins or triol group saponins to acidic amino acids is 1:10 (kg / kg); then put it in a pressure cooker and cook at 80°C for 1 hour, take out the cooked hydrolyzate and put it on the macroporous resin column D 101 , first washed with water for five column volumes, and then eluted with 60% ethanol. After recovering ethanol, the hydrolyzed product of ginseng diol group saponins obtained rare ginsenoside 20R-Rg 3 、20S-Rg 3 , Rg 5 and Rk1( figure 1 and image 3 ); Panaxatriol group saponin hydrolyzate obtains rare ginsenoside Rg 6 , F4, Rk3 and Rh4 ( figure 2 and Figure 4 ).

Embodiment 2

[0022] Panaxadiol group saponins or triol group saponins are dissolved in water according to the mass volume ratio of 1:10 (kg / liter), and acidic amino acids are added, so that the mass ratio of panaxadiol group saponins or triol group saponins to acidic amino acids is 1:1 (kg / kg); then put it in a pressure cooker and cook at 100°C for 2 hours, take out the cooked hydrolyzate and put it on the macroporous resin column D 101 , first washed with water for five column volumes, and then eluted with 70% ethanol. After recovering ethanol, the hydrolyzed products of ginsenosides of ginseng diol group obtained rare ginsenoside 20R-Rg 3 、20S-Rg 3 , Rg 5 and Rk1( figure 1 and image 3 ); Panaxatriol group saponin hydrolyzate obtains rare ginsenoside Rg 6 , F4, Rk3 and Rh4 ( figure 2 and Figure 4 ).

Embodiment 3

[0024] Panaxadiol group saponins or triol group saponins are dissolved in water according to the mass volume ratio of 1:20 (kg / liter), and acidic amino acids are added, so that the mass ratio of panaxadiol group saponins or triol group saponins to acidic amino acids is 20:1 (kg / kg); then put it in a pressure cooker and cook at 120°C for 4 hours, take out the cooked hydrolyzate and put it on the macroporous resin column D 101 , first washed with water for five column volumes, and then eluted with 80% ethanol. After recovering ethanol, the hydrolyzed products of ginsenosides of ginseng diol group obtained rare ginsenoside 20R-Rg 3 、20S-Rg 3 , Rg 5 and Rk1( figure 1 and image 3 ); Panaxatriol group saponin hydrolyzate obtains rare ginsenoside Rg 6 , F4, Rk3 and Rh4 ( figure 2 and Figure 4 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing rare ginsenosides by hydrolyzing ginsenosides with acidic amino acids. The saponins of the ginseng diol group are transformed into 20R‑Rg3, 20S‑Rg3, Rg5 and Rk1, and the saponins of the ginseng triol group are transformed into Rg6, F4, Rk3 and Rh4. Compared with the existing preparation process, the hydrolysis ability of acidic amino acid is more than 10 times that of neutral amino acid and basic amino acid, which is more suitable for the needs of industrial production of rare ginseng saponins. Poor consistency, low yield, strong corrosion, polluting the environment and many by-products, etc.; making full use of ginseng resources, maximally extracting and enriching rare ginseng saponins, and achieving simple, rapid, environmentally friendly, and low-cost enrichment The purpose is to provide method assurance for industrial production and new drug preparation.

Description

technical field [0001] The invention discloses a method for preparing rare ginsenosides by hydrolyzing ginsenosides with acidic amino acids, and relates to ginsenoside 20R-Rg 3 、20S-Rg 3 , Rk1, Rg 5 , Rg 6 , F4, Rk3 and Rh4 extraction, belonging to the technical field of extraction and separation of effective components of traditional Chinese medicine. Background technique [0002] In the technical field of ginseng preparation, ginsenosides are divided into diol type ginsenosides (PPD) and triol type ginsenosides (PPT) according to the structure of aglycones. Both PPD and PPT ginsenosides belong to dammarane-type tetracyclic triterpenoids, which account for the majority of ginsenosides. Rare ginsenosides, such as ginsenoside Rg 3 , Rg 5 , F 4 , RK1, Rh 2 、Rh 4 etc., exist or not exist in trace amounts in ginseng. The latest pharmacological studies have shown that rare ginsenosides have a variety of physiological activities, such as anti-tumor, immune regulation, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00
Inventor 刘志阮长春孙光芝张金秋夏娟
Owner JILIN YATAI PHARM CO LTD