Nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, production method and application

A technology of Nitrobacter victoria and compound bacterial agents, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the difficulty of increasing the production cost and application of bacterial agents, and can not achieve simple mixed fermentation Composite, industrial production difficulties and other problems, to achieve the effect of shortening the fermentation time, shortening the cultivation time, and reducing production costs

Active Publication Date: 2016-01-27
WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because nitrifying bacteria are inorganic chemoautotrophic bacteria, their growth is slow, industrial production is difficult, and a single nitrifying bacteria cannot completely remove nitrogen from water, and a large amount of total nitrogen is often accumulated during the application process. In order to effectively eliminate Total nitrogen, people often achieve denitrification by adding denitrifying bacteria in the later stage
At present, the normal autotrophic nitrifying bacteria are all liquid bacterial agents, and the denitrifying bacteria are solid bacterial agents. The two bacterial agents are produced by different production processes, and it is impossible to achieve mixed fermentation or simple compounding. The two types of bacterial agents are produced separately. And the application increases the production cost and the difficulty of the application of the bacterial agent to a certain extent

Method used

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  • Nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, production method and application
  • Nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, production method and application
  • Nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, production method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A kind of Nitrobacter victoria Y3-2 obtained by:

[0047] The strain was obtained by enrichment, separation and purification from the activated sludge of Wuhan Longwangmiao Sewage Treatment Plant in 2008 by the inventor. The strain was sent to the China Center for Type Culture Collection on May 14, 2014 for preservation, with a classification name: Nitrobacter Winogradskyi (Nitrobacter Winogradskyi) Y3-2, a preservation number: CCTCCNO: M2014203, and an address: Wuhan University, Wuhan.

[0048] The method for cultivating the bacterial agent alone adopts a nitrifying bacteria medium for cultivating, with a pH of 8.0-8.5, a temperature of 30° C., an inoculation amount of 10%, and 200 rpm shaker culture. The medium formula is as follows: NaCl0.3g / L, MgSO 4 ·7H 2 O0.04g / L, FeSO 4 0.0396g / L, NaNO 2 0.5g / L, K 2 HPO 4 ·3H 2 O4.108g / L, KH 2 PO 4 0.272g / L.

Embodiment 2

[0050] A kind of application of Nitrobacter victoria Y3-2:

[0051] (1) Application of Nitrobacter victoria Y3-2 in aquaculture fish tanks

[0052] Fill 14L of water in a 20L glass fish tank, raise 6 small red goldfish (about 6g / tail), add 1.2g of fish feed every day, keep a constant temperature of 25°C during the breeding process, and continue to ventilate; Accumulation to 2.0-2.2mg / l is not conducive to the growth of fish. Set up the bacterial injection group and the control group, each with 3 parallels, and the bacterial injection group was added with Nitrobacter verdeii Y3-2, the bacterial concentration was 1.5×10 8 cfu / ml, the dosage is 2.8ml, and it is only added once on the first day; the control group does not inject bacteria. Detect NO in the water body every day 2 -N, NO 3 -N concentration changes, the results are shown in Table 1 below.

[0053] Table 1, the application effect of Nitrobacter victoria Y3-2 in aquaculture fish tanks (unit: mg / L)

[0054]

[0...

Embodiment 3

[0063] The invention relates to a nitrifying bacteria-denitrifying bacteria bacterial agent, which includes Nitrosomonas europeanus, Nitrobacter victoria and Pseudomonas stutzeri.

[0064] The ratio of each component of the bacterial agent is: Nitrosomonas europeanus: Nitrobacter victoria: Pseudomonas stutzeri is 1 × 10 7 -5×10 8 cfu / ml: 1×10 7 -5×10 8 cfu / ml: 1×10 8 -1×10 9 cfu / ml.

[0065] The N. europeanus is ATCC19718.

[0066] Nitrobacter victorii is Nitrobacter victorii Y3-2, CCTCC NO: M2014203.

[0067] Pseudomonas stutzeri is ATCC17588.

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Abstract

The invention discloses nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, a production method and application. Nitrobacter winogradsky is deposited at the China Center for Type Culture Collection with the accession number of ((i) Nitrobacter Winogradsky i (/i)) Y3-2, CCTCC NO: M2014203. The nitrobacter winogradsky has a function of effectively treating nitrite, and is mixed with nitrosomonas europaea and pseudomonas stutzeri to obtain the complex microbial inoculants, and the complex microbial inoculants can efficiently remove nitrogen in water, and can be used for treating aquaculture water, livestock breeding sewage or industrial sewage.

Description

technical field [0001] The invention relates to the field of microorganisms and environmental engineering, in particular to a Nitrobacter victorii and a nitrifying bacteria-denitrifying bacteria composite bacterial agent, a production method and an application thereof. Background technique [0002] Water, as a resource that human beings rely on for survival, is in constant pollution and urgently needs to be treated at the same time. As bioremediation methods are increasingly valued by humans, the application of nitrifying bacteria and denitrifying bacteria has gradually risen to a new level. [0003] Microbial denitrification is mainly realized through the process of nitrification-denitrification, in which the main undertaker of the nitrification process is nitrifying bacteria, and the main undertaker of the denitrification process is denitrifying bacteria. Nitrifying bacteria are divided into two categories: ammonia oxidizing bacteria and nitrite oxidizing bacteria. They c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38C02F101/38
Inventor 吴定心梁运祥周闯林彦徐
Owner WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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