A kind of Nitrobacter victoria and nitrifying bacteria-denitrifying bacteria composite bacterial agent and its production method and application
A technology of Nitrobacter victoria and denitrifying bacteria, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the difficulty of increasing the production cost and application of bacterial agents, and can not achieve simple mixed fermentation Composite, industrial production difficulties and other problems, to achieve the effect of shortening the fermentation time, shortening the cultivation time, and reducing production costs
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Embodiment 1
[0046] A kind of Nitrobacter victoria Y3-2 obtained by:
[0047] The strain was obtained by enrichment, separation and purification from the activated sludge of Wuhan Longwangmiao Sewage Treatment Plant in 2008 by the inventor. The strain was sent to the China Center for Type Culture Collection on May 14, 2014 for preservation. The taxonomic name: Nitrobacter Winogradskyi (Nitrobacter Winogradskyi) Y3-2, preservation number: CCTCC NO: M2014203, address: Wuhan University, Wuhan.
[0048] The method for cultivating the bacterial agent alone adopts a nitrifying bacteria culture medium for culturing, with a pH of 8.0-8.5, a temperature of 30° C., an inoculum size of 10%, and 200 rpm shaker culture. The medium formula is as follows: NaCl 0.3g / L, MgSO 4 ·7H 2 O 0.04g / L, FeSO 4 0.0396g / L, NaNO 2 0.5g / L, K 2 HPO 4 ·3H 2 O 4.108g / L, KH 2 PO 4 0.272g / L.
Embodiment 2
[0050] A kind of application of Nitrobacter victoria Y3-2:
[0051] (1) Application of Nitrobacter victoria Y3-2 in aquaculture fish tanks
[0052] Fill 14L of water in a 20L glass fish tank, raise 6 small red goldfish (about 6g / tail), add 1.2g of fish feed every day, keep a constant temperature of 25°C during the breeding process, and continue to ventilate; Accumulation to 2.0-2.2mg / l is not conducive to the growth of fish. Set up the bacterial injection group and the control group, each with 3 parallels, and the bacterial injection group was added with Nitrobacter verdeii Y3-2, the bacterial concentration was 1.5×10 8 cfu / ml, the dosage is 2.8ml, and it is only added once on the first day; the control group does not inject bacteria. Detect NO in the water body every day 2 -N, NO 3 -N concentration changes, the results are shown in Table 1 below.
[0053] Table 1, the application effect of Nitrobacter victoria Y3-2 in aquaculture fish tanks (unit: mg / L)
[0054]
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Embodiment 3
[0063] The invention relates to a nitrifying bacteria-denitrifying bacteria bacterial agent, which includes Nitrosomonas europeanus, Nitrobacter victoria and Pseudomonas stutzeri.
[0064] The ratio of each component of the bacterial agent is: Nitrosomonas europeanus: Nitrobacter victoria: Pseudomonas stutzeri is 1 × 10 7 -5×10 8 cfu / ml: 1×10 7 -5×10 8 cfu / ml: 1×10 8 -1×10 9 cfu / ml.
[0065] The N. europeanus is ATCC19718.
[0066] Nitrobacter victorii is Nitrobacter victorii Y3-2, CCTCC NO: M 2014203.
[0067] Pseudomonas stutzeri is ATCC17588.
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