Recombinant microorganisms exhibiting increased flux through a fermentation pathway

A technology for recombining microorganisms and microorganisms, applied in the field of identifying and solving reaction bottlenecks in fermentation pathways, and can solve problems such as inability to identify rate-limiting reactions
CN105283554AInactive Publication Date: 2016-01-27LANZATECH NEW ZEALAND LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LANZATECH NEW ZEALAND LTD
Publication Date
2016-01-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides methods of increasing the production of fermentation products by increasing flux through a fermentation pathway by optimising enzymatic reactions. In particular, the invention relates to identifying enzymes and / or co-factors involved in metabolic bottlenecks in fermentation pathways, and fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism adapted to exhibit increased activity of the one or more of said enzymes, or increased availability of the one or more of said co-factors, when compared to a parental microorganism.
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Description

[0001] Cross References to Related Applications

[0002] This application claims priority to Provisional Application No. 61 / 831,591, filed June 5, 2013, the contents of which are hereby incorporated by reference. technical field

[0003] The present invention relates to methods of increasing flux through fermentation pathways by optimizing enzymatic reactions. More specifically, the invention relates to identifying and resolving reaction bottlenecks in fermentation pathways. Background technique

[0004] Acetogenic microorganisms are known to be useful in the production of fuels and other chemicals such as ethanol, butanol or butanediol by fermenting substrates including, for example, carbon monoxide, carbon dioxide, and hydrogen.

[0005] Efforts to improve product concentration and substrate utilization to date have focused on strain selection, optimization of fermentation conditions and parameters, and optimization of media formulation or process conditions (Abubackar e...

Claims

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