Method for removing bicuculline from rhizoma corydalis decumbentis total alkaloids, product and application

A technology of total alkaloids and inorganic bases, applied in medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve the problem of high content of bikokoling in summer without total alkaloids, which is not suitable for clinical application and industrialization Production and other issues

Active Publication Date: 2016-02-10
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a summer free total alkaloid bikkoling content in the prior art, which is not suitable for clinical application and industrial production. The removal method, product and application of Koukouling

Method used

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  • Method for removing bicuculline from rhizoma corydalis decumbentis total alkaloids, product and application
  • Method for removing bicuculline from rhizoma corydalis decumbentis total alkaloids, product and application
  • Method for removing bicuculline from rhizoma corydalis decumbentis total alkaloids, product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The summer non-supercritical total alkaloid extract was formulated into dehydrated ethanol solutions with concentrations of 0.05g / mL, 0.02g / mL, 0.01g / mL, and 0.002g / mL, respectively. It was found that the 0.05g / mL summer non-supercritical extract solution contained many oily insolubles, which could not be completely dissolved under the condition of heating and ultrasonic. Take 20 mL of each concentration solution, and add 5 mL to 10 mL of 0.5 mol / L NaOH aqueous solution in 0.05 g / mL, 0.02 g / mL, 0.01 g / mL, 0.002 g / mL summer non-supercritical extract anhydrous ethanol solution respectively , stirred and allowed to stand overnight. The next day, dry under reduced pressure, add 25 mL of double-distilled water, and mix well by ultrasonic. At room temperature, transfer to a separatory funnel, and extract 3 times with 25 mL of ethyl acetate until colorless. After spin-drying, take 0.05g, dissolve it with mobile phase and dilute it to 25mL, inject it into the chromatograph re...

Embodiment 2

[0079] Take 3 portions of 0.5g summer non-supercritical total alkaloid extract, dissolve them in 25mL of absolute ethanol, and add 0.1mol / L NaOH, 0.5mol / LNaOH, 0.8mol / LNaOH 5mL to 10mL respectively after completely dissolving. Let stand overnight, dry under reduced pressure, add double distilled water, and mix well by ultrasonic. At room temperature, transfer to a separatory funnel, and extract 3 times with an equal amount of ethyl acetate until colorless. After being spin-dried, they were respectively injected into a chromatograph for analysis. When the NaOH is about 0.5mol / L-0.8mol / L, the reaction can be completed, and the specific data are shown in Table 2.

[0080] The impact table of the concentration of table 2 alkali on the removal rate of Bikoukouling

[0081] Different alkali concentration (mol / L)

Embodiment 3

[0083] Take 3 parts of 0.5g summer non-supercritical total alkaloid extract, dissolve them in 25mL of absolute ethanol respectively, after completely dissolving, add 5mL-10mL0.5mol / NaOH respectively, react at room temperature for 0.5h, 1h, 2h and spin dry. Add double distilled water and mix by ultrasonic. At room temperature, transfer to a separatory funnel, and extract 3 times with an equal amount of ethyl acetate until colorless. After being spin-dried, it was injected into a chromatograph for analysis. When the reaction time is greater than 1 h, the content of bikokoling is relatively low, and the reaction is relatively complete. The specific data are shown in Table 3.

[0084] Table 3 The impact table of the time of hydrolysis reaction on bikokoling removal rate

[0085] different reaction times

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Abstract

The invention discloses a method for removing bicuculline from rhizoma corydalis decumbentis total alkaloids, a product and application. The method for removing the bicuculline from the rhizoma corydalis decumbentis total alkaloids includes the steps of (1) subjecting supercritical extract of rhizoma corydalis decumbentis and alkali to hydrolysis reaction in solvents so as to obtain the rhizoma corydalis decumbentis total alkaloids; (2) separating the rhizoma corydalis decumbentis total alkaloids, obtained in the step (1), in a high-speed centrifugal partition chromatographic instrument by high-speed centrifugal partition chromatography so as to obtain rhizoma corydalis decumbentis total alkaloids with the bicuculline removed. The method for removing the bicuculline from the rhizoma corydalis decumbentis total alkaloids is simple in steps, short in time, high in removal rate and suitable for large-scale industrial production, and the rhizoma corydalis decumbentis total alkaloids obtained by the method are low in bicuculline content and suitable for clinical application.

Description

technical field [0001] The invention relates to a removal method, product and application of Bikoukouling in summer free total alkaloids. Background technique [0002] Cerebrovascular disease is a very serious health and social problem, characterized by high morbidity, high disability, high recurrence, high mortality, and high proportion of medical expenses, and it is still on the rise. In European and American countries, cerebrovascular disease is the third leading cause of death after tumor and heart disease; in China, cerebrovascular disease is the second leading cause of death after tumor. The representative of cerebrovascular disease is stroke. About 80% of strokes belong to ischemic stroke, which refers to the disorder of blood supply to the brain caused by various reasons, or the reduction of arterial blood perfusion, or the sudden complete interruption of blood flow to stop the blood supply, resulting in brain tissue ischemia and hypoxia. Necrosis, irreversible dam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P9/10A61P25/00
Inventor 杨义芳谢欣辛孙百玲
Owner SHANGHAI INST OF PHARMA IND CO LTD
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