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Composite polypeptide additive feed and making method thereof

A production method and additive technology, applied in animal feed, animal feed, application, etc., can solve problems such as obvious side effects

Inactive Publication Date: 2016-02-17
SHANGHAI YANHUA FEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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However, its side effects are also particularly obvious, es

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  • Composite polypeptide additive feed and making method thereof
  • Composite polypeptide additive feed and making method thereof

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Embodiment Construction

[0017] In order to facilitate the understanding of the structure of the present invention, the following will be described in conjunction with the examples.

[0018] The invention provides a composite polypeptide additive feed, which includes antimicrobial peptide fragments, and the antimicrobial peptide fragments are formed by purifying and concentrating yeast genes. The formulation materials of the yeast genes include: 54% corn, 16.3% soybean meal, % green bran, 0.04% DDGS, 0.05% cotton meal, 0.03% layer premix, 0.06% stone powder, 0.02% shell meal and 0.01% fish meal.

[0019] 1. Gene expression method to obtain effective antimicrobial peptide substances:

[0020] Yeast is used as the genetically engineered recipient bacteria to express. Yeast has a more complete gene expression regulation mechanism than E. coli and the ability to process, modify and secrete the expressed product, does not produce endotoxin, and uses electrophoresis technology to obtain antimicrobial peptid...

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Abstract

The invention provides a composite polypeptide additive feed and a making method thereof. The feed comprises an antibacterial peptide fragment which is formed through purification and concentration of a yeast gene. The making method comprises the following steps: chemically synthesizing an antibacterial peptide magainin gene fragment coded by a yeast preference codon; recombining the antibacterial peptide bombesin gene fragment with a yeast expression vector so as to form an intracellular expression vector; transforming a host bacterium in the expression vector by electroporation; subjecting the antibacterial peptide magainin gene fragment to purification and concentration; and mixing the purified and concentrated antibacterial peptide magainin gene fragment with conventional accessory materials so as to form the composite polypeptide additive feed. According to the invention, a novel antibiotic substitute is formed; dependence and abuse of antibiotics are reduced; and sound development of the livestock and poultry industry is promoted.

Description

technical field [0001] The invention specifically relates to a compound polypeptide additive feed and a preparation method thereof. Background technique [0002] Due to the strong antibacterial and bactericidal effects of antibiotics, they are widely used all over the world, and play an important role in the treatment of animal diseases, improving feed utilization, and promoting the growth of livestock and poultry. However, its side effects are also particularly obvious, especially in aspects such as bacterial drug resistance and drug residues. In 2006, the European Union completely banned the use of antibiotics as feed additives. [0003] Antimicrobial peptides originally refer to a class of basic polypeptide substances with antibacterial activity induced in insects. Its molecular weight is about 2000-7000, and it is composed of 20-60 amino acid residues. Most of these active polypeptides have the characteristics of strong alkalinity, thermal stability and broad-spectrum ...

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Application Information

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IPC IPC(8): A23K1/16
Inventor 张泽宏
Owner SHANGHAI YANHUA FEED CO LTD
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