Tissue culture rapid propagation method for cerasus yunnanensis(Franch.)Yu et Li adult excellent single plant 'Guangzhou' cerasus yunnanensis

A tissue culture rapid propagation and excellent technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of tissue culture buds yellowing and dropping leaves, poor quality of seedlings, and few effective buds, etc. The effect of strong rooting seedlings, short cultivation period and fast induction speed

Active Publication Date: 2016-02-24
GUANGZHOU TRENDSEE GRP +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the tissue culture and rapid propagation of 'Guangzhou' cherry is carried out with reference to the tissue culture medium of the related species of the genus Prunus, the tissue culture buds will turn yellow and drop leaves, elongation and growth will be slow, and finally the phenomenon of withering and yellowing will appear. The buds are small and weak, which is effective. The number of buds is small, resulting in small multiplication coefficient, poor quality of seedlings, slow propagation speed and high production cost

Method used

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  • Tissue culture rapid propagation method for cerasus yunnanensis(Franch.)Yu et Li adult excellent single plant 'Guangzhou' cerasus yunnanensis
  • Tissue culture rapid propagation method for cerasus yunnanensis(Franch.)Yu et Li adult excellent single plant 'Guangzhou' cerasus yunnanensis
  • Tissue culture rapid propagation method for cerasus yunnanensis(Franch.)Yu et Li adult excellent single plant 'Guangzhou' cerasus yunnanensis

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Experimental program
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Effect test

Embodiment 1

[0028] A method for tissue culture and rapid propagation of an excellent adult single plant 'Guangzhou' cherry of Yunnan cherry, comprising the following steps:

[0029] (1) Explant collection: From March to May, when the weather is clear at about 10 am, the semi-lignified shoots with robust growth and no pests and diseases were cut from the mother plant of 'Guangzhou', and brought back to the laboratory after moisturizing treatment. The collected semi-lignified shoots were defoliated, washed with purified water, and stored as explants in a 4°C refrigerator for later use. Before collecting explants, sprout shoots were sprayed alternately with carbendazim and chlorothalonil 4 to 5 times every 7 days.

[0030] (2) Surface disinfection of explants: Cut the branches (explants) into stems with leaf axils about 4cm long, wash them with sterile water on the ultra-clean workbench, and then use a mass fraction of 0.5% Xinjier The sterilization solution, the soaking time is 6min, after...

Embodiment 2

[0040] A method for tissue culture and rapid propagation of an excellent adult single plant 'Guangzhou' cherry of Yunnan cherry, comprising the following steps:

[0041] (1) Explant collection: From March to May, when the weather is clear at about 10 am, the semi-lignified shoots with robust growth and no pests and diseases were cut from the mother plant of 'Guangzhou', and brought back to the laboratory after moisturizing treatment. The collected semi-lignified shoots were defoliated, washed with purified water, and stored as explants in a 4°C refrigerator for later use. Before collecting explants, sprout shoots were sprayed alternately with carbendazim and chlorothalonil 4 to 5 times every 7 days.

[0042] (2) Disinfection of the explant surface: Cut the branches (explants) into stems with leaf axils about 4cm long, wash them with sterile water on the ultra-clean workbench, and then use 1% Xinjier by mass fraction. The sterilizing solution, the soaking time is 5min, after p...

Embodiment 3

[0052] A method for tissue culture and rapid propagation of an excellent adult single plant 'Guangzhou' cherry of Yunnan cherry, comprising the following steps:

[0053] (1) Explant collection: From March to May, when the weather is clear at about 10 am, the semi-lignified shoots with robust growth and no pests and diseases were cut from the mother plant of 'Guangzhou', and brought back to the laboratory after moisturizing treatment. The collected semi-lignified shoots were defoliated, washed with purified water, and stored as explants in a 4°C refrigerator for later use. Before collecting explants, sprout shoots were sprayed alternately with carbendazim and chlorothalonil 4 to 5 times every 7 days.

[0054] (2) Surface disinfection of explants: Cut the branches (explants) into stems with leaf axils about 4cm long, wash them with sterile water on the ultra-clean workbench, and then use a mass fraction of 0.5% Xinjier The sterilization solution, the soaking time is 6min, after...

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Abstract

The invention discloses a tissue culture rapid propagation method for a cerasus yunnanensis(Franch.)Yu et Li adult excellent single plant 'Guangzhou' cerasus yunnanensis. The method comprises arranging a basal medium, optimizing different hormone compositions and concentration level ratio, taking an adult excellent plant annual semi-lignified branch as an explant, performing axillalry bud induction, axillalry bud propagation and rooting culture, so as to form a complete plant, and transplanting to a matrix, so as to obtain tidily-grown robust nursery stocks with consistent phenotype. The method possesses the advantages of high propagation coefficient, short culture period, no season restriction and the like. The inductivity reaches 100%, the propagation coefficient reaches 4.72. The propagation bud stretches fast and grows fast, and possesses the height of 3-4 cm after being cultured for 15 d. After rooting culture is performed for 15 d, the rooting rate reaches 100%, and the root number in per one plant reaches five or more. The tissue-culture rooted seedling is robust and possesses the transplantation survival rate up to 90% or more. During practical production application, the method can be used for one-year large-scale rapid seedling growing, and robust tidy consistent 'Guangzhou' cerasus yunnanensis seedlings are produced, and the method possesses wide application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture and rapid propagation method for a fine adult single plant of Yunnan cherry 'Guangzhou' cherry. Background technique: [0002] Yunnan cherry (Cerasus yunnanensis (Franch.) Yuet Li) is a deciduous tree of the genus Cerasus in the family Rosaceae. It is native to Yunnan, Sichuan, and Guangxi in valley forests or hillsides at an altitude of 2300-2600m. The flowers are white, in a raceme near the corymb, and the flowers and leaves open together or the flowers open near the first leaves. Drupe purple. The flowering period in the place of origin is March to May, and the fruiting period is May to June. It is an important landscaping tree species in the place of origin, widely planted in parks, scenic spots, schools, residential quarters, and both sides of urban roads. 'Guangzhou' cherry is an excellent fast-growing single plant selected fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 何宗儒邓小梅叶小玲奚如春胡晓敏刘湘源唐伟洲
Owner GUANGZHOU TRENDSEE GRP
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