A kind of pharmaceutical composition and its application
A composition and drug technology, applied in the direction of drug combination, non-active ingredients of polymer compounds, anti-tumor drugs, etc., can solve the problems of different molecular properties, achieve significant drug efficacy, and inhibit tumor growth
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Embodiment 1
[0059] Example 1. Agarose gel electrophoresis experiment analysis of the appropriate nitrogen and phosphorus mass ratio (N / P) of HKP and miR-150
[0060] Agarose gel electrophoresis is an electrophoresis method using agarose as a supporting medium, which has the dual functions of "molecular sieve" and "electrophoresis". The separation of charged particles depends not only on the nature and quantity of the net charge, but also on the size of the molecule. It is a common method for nucleic acid separation and detection, and 1% agarose is commonly used as an electrophoresis support. In this experiment, 1% agarose gel was used to observe the loading capacity of HKP on micro RNA.
[0061] HKP uses two outsourcing companies from Mixson and API, which synthesize HKP according to the technology owned by the patentee, and conduct the pre-screening test of nitrogen and phosphorus mass ratio with Mimics respectively, setting the following five ratios: 1:1, 2:1, 4 :1, 6:1, 8:1.
[0062]...
Embodiment 2
[0063] Example 2. Experimental Analysis of Particle Size and Potential Data Measurement Appropriate Nitrogen-Phosphorus Mass Ratio (N / P) of HKP and miR-150
[0064] Dynamic Light Scattering (DLS) measurement of particle size has the advantages of accuracy, speed, and good repeatability, and has become a relatively routine characterization method in nanotechnology. The laser particle size analyzer using the principle of dynamic light scattering can directly measure the particle size distribution of the nanoparticle suspension.
[0065] Zeta potential, also known as zeta potential or zeta potential (ζ-potential or ζ-potential), refers to the potential of the shear plane (ShearPlane), and is an important indicator to characterize the stability of nano-solution dispersion system. It is the potential difference between the continuous phase and the fluid stable layer attached to the dispersed particles, which can be directly measured by electrokinetic phenomena. A zeta potentiomete...
Embodiment 3
[0071] Example 3. Encapsulation Efficiency Determination Experimental Analysis of the Appropriate Nitrogen-Phosphorus Mass Ratio (N / P) of miR-150 and HKP
[0072] RiboGreen RNA Quantitation Reagent is an ultrasensitive nucleic acid fluorescent dye that allows simple and rapid quantification of RNA in solution.
[0073] When RiboGreen fluorescent dye is in a solution state, it has almost no fluorescence activity; when RiboGreen is combined with RNA, its fluorescence activity will increase by 1000 times. The fluorescence excitation wavelength of RiboGreen-RNA complex is about 500nm, and the emission wavelength is about 525nm.
[0074] When the RibGreen fluorescent dye is added to the solution, the free miRNA will bind to it, forming a RiboGreen-RNA complex with fluorescent activity. However, the miRNA encapsulated by HKP will not bind to the RibGreen fluorescent dye and will not produce fluorescent activity. Therefore, by drawing the standard curve with the fluorescence degree...
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