Tripterygium hypoglaucum hutch active-components detection method based on ionic liquid-ultrasonic assistance-HPLC

An ultrasonic-assisted, ionic liquid technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of difficult detection of diverse active components, and achieve the effect of avoiding detection errors

Inactive Publication Date: 2016-03-02
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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Problems solved by technology

[0004] In order to solve the problem of the difficulty in detecting the diversity of active ingredients in Begonia Kunming, the present invention provides a method for detecting the active components of Begonia Kunming based on ionic liquid-ultrasound-assisted-HPLC, wherein the key technology is that the prepared samples are Using the solution of 1-butyl-3-methylimidazolium hexafluorophosphate as the extraction solution to extract Kunming mountain crabapple

Method used

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  • Tripterygium hypoglaucum hutch active-components detection method based on ionic liquid-ultrasonic assistance-HPLC
  • Tripterygium hypoglaucum hutch active-components detection method based on ionic liquid-ultrasonic assistance-HPLC
  • Tripterygium hypoglaucum hutch active-components detection method based on ionic liquid-ultrasonic assistance-HPLC

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Embodiment 1

[0052] The condition investigation of embodiment 1HPLC

[0053] 1. Instruments and reagents

[0054] Shimadzu HPLCLC-30A high-performance liquid chromatograph, AEG-45SM electronic balance (1 / 100,000, Shimadzu Corporation of Japan), BP121S (1 / 10,000) (Beijing Sartorius Scientific Instrument Co., Ltd.). KQ250DB CNC ultrasonic cleaner (power 250W, frequency 40kHz, Gongyi Yuhua Instrument Co., Ltd.), BJ-100 multifunctional pulverizer (Deqing Baijie Electric Co., Ltd.),

[0055] 1-Butyl-3-methylimidazolium bromide ([BMIm]Br, purity ≥99%), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm]BF 4 , purity ≥ 99%), 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF 6 , purity ≥ 99%), 1-hexyl-3-methylimidazolium tetrafluoroborate ([HMIm]BF 4 , purity ≥99%), 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIm]PF 4, purity ≥ 99%), 1-octyl-3-methylimidazolium tetrafluoroborate ([OMIm] BF4, purity ≥ 99%), all provided by Lanzhou Institute of Chemical Physics, Chinese Academy ...

Embodiment 2

[0064] The investigation of embodiment 2 preparation sample conditions

[0065] 1. Investigation of the types of ionic liquids in the extraction conditions

[0066] Take 0.5g each of 7 portions of Kunming Begonia powder (passed through a No. 3 sieve), weigh them accurately, and add 0.6mol·L -1[15] Six ionic liquids: 1-butyl-3-methylimidazolium bromide ([BMIm]Br), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm]BF 4 ), 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF 6 ), 1-hexyl-3-methylimidazolium tetrafluoroborate ([HMIm]BF 4 ), 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIm]PF 6 ), 1-octyl-3-methylimidazolium tetrafluoroborate ([OMIm]BF4)﹜ methanol solution and pure methanol solvent 2mL each, ultrasonic extraction for 30min, centrifugation for 5min (12000r·min -1 ), filtered through a microporous membrane, injected into a high-performance liquid chromatograph, recorded the peak area, calculated the extraction rate of each component, investigated th...

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Abstract

The invention provides a tripterygium hypoglaucum hutch active-components detection method based on ionic liquid-ultrasonic assistance-HPLC. The key technology is that a preparation sample 1-butyl-3-methylimidazolehexafluorophosphate solution is used as an extracting solution to extract a tripterygium hypoglaucum hutch medicinal material. Detection efficiency of the method provided by the invention is obviously higher than detection efficiency of a traditional single-component detection method. The more important is that transfer rate of active components in the process is obviously higher than transfer rate of a traditional methanol extraction method. And detection errors are effectively avoided.

Description

technical field [0001] The invention relates to a chromatographic detection method of Chinese medicinal materials, and more specifically relates to a detection method of Kunming mountain crabapple active components based on ionic liquid-ultrasound-assisted-HPLC. technical background [0002] Kunming Begonia is the dried root of Tripterygium hypoglaucum (Levl.) Hutch., a plant of the Euonymus family Tripterygium. . Also known as torch flower, heartbroken grass, purple gold skin, etc., the name of torch flower was first recorded in the "Compendium of Materia Medica". The raw material of "Begonia Tablets" is also a medicinal plant of Tripterygium wilfordii with high efficacy and low toxicity to humans and animals. It has a wide range of applications and has anti-inflammatory, immunosuppressive, anti-tumor, and kidney function improvement functions. It has been used for insecticide since ancient times. , relaxing tendons and activating collaterals, expelling wind and dehumidif...

Claims

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Application Information

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IPC IPC(8): G01N30/14
Inventor 陈一龙张小梅励娜姚媛媛梁旭明杨大坚张毅祝卢艺
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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