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SOD (superoxide dismutase) ethosome and preparation method thereof

A technology of ethosomes and plastids, which is applied in the field of SOD ethosomes and its preparation, can solve the problems of easy to be digested by enzymes, immunogenicity, short biological half-life, poor stability of SOD, etc., and achieve good skin penetration and improve stability The effect of sex, method and process is simple

Inactive Publication Date: 2016-03-09
AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor stability of SOD at room temperature, short biological half-life, easy to be hydrolyzed and immunogenicity, its clinical application is limited.

Method used

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  • SOD (superoxide dismutase) ethosome and preparation method thereof

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Effect test

Embodiment 2

[0028] The preparation method of the SOD ethosome of Example 2: 100 mg of galactosylceramide was dissolved in 6 ml of a mixed solution of ethanol and propylene glycol with a mass ratio of 6:5 to obtain solution A, and 40 mg of PEG-modified SOD was dissolved in 4 ml of In the ethanol solution and stirred evenly to obtain solution B, mix solution A and solution B, stir evenly with a magnetic stirrer at a constant temperature of 20-40°C and a stirring speed of 100rpm, after forming a uniform milky white suspension, add buffer Liquid PBS, pH 8, then probe-type ultrasonic treatment under ice bath conditions, and finally pass through 0.45um and 0.22um filter membranes successively to obtain SOD ethosomes.

Embodiment 3

[0029] The preparation method of the SOD ethosome of Example 3: 300 mg of galactosylceramide was dissolved in 6 ml of a mixed solution of ethanol and propylene glycol with a mass ratio of 7:3 to obtain solution A, and 20 mg of PEG-modified SOD was dissolved in 3 ml of volume 40% ethanol solution and stir evenly to obtain solution B, mix solution A and solution B, stir evenly with a magnetic stirrer at a constant temperature of 20-40°C and a stirring speed of 100rpm, until a uniform milky white suspension is formed After mixing, add buffer solution PBS, the pH is 8, then use probe-type ultrasonic treatment under ice bath conditions, and finally pass through 0.45um and 0.22um filter membranes successively to obtain SOD ethosomes.

Embodiment 4

[0030] The preparation method of the SOD ethosome of Example 4: 200mg of galactosylceramide was dissolved in 3ml of ethanol and propylene glycol mixed solution with a mass ratio of 6:5 to obtain solution A, and 30mg of PEG-modified SOD was dissolved in 2ml volume 40% ethanol solution and stir evenly to obtain solution B, mix solution A and solution B, stir evenly with a magnetic stirrer at a constant temperature of 20-40°C and a stirring speed of 100rpm, until a uniform milky white suspension is formed After mixing, add buffer solution PBS, the pH is 8, then use probe-type ultrasonic treatment under ice bath conditions, and finally pass through 0.45um and 0.22um filter membranes successively to obtain SOD ethosomes.

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Abstract

The invention discloses an SOD (superoxide dismutase) ethosome and a preparation method thereof. The ethosome mainly consists of the following components: galactosyl ceramide, PEG (polyethylene glycol) modified SOD, low-molecule alcohol and water; and the preparation method mainly comprises the following steps: according to constituting proportions of the components, dissolving the galactosyl ceramide in a low-molecule alcohol mixed solution so as to obtain a solution A; dissolving the PEG modified SOD in ethanol and uniformly stirring so as to obtain a solution B; mixing the solution A with the solution B; adding a buffer solution as a uniform milk white suspension is formed; then, ultrasonically processing in an ice-bath condition; and finally, processing by virtue of a filtering membrane, so that the SOD ethosome is obtained. The SOD ethosome has the advantages of strong stability, strong transdermal penetration and simple preparation process.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a SOD ethosome and a preparation method thereof. Background technique [0002] For many topical drugs for skin, the deep layer of skin tissue, epidermis and dermis are all the target tissues for their action. Because the stratum corneum hinders the transmission of drugs, it is difficult for topical drugs to reach the depth of action. At present, there are many studies on carrier liposomes for transdermal drug delivery. [0003] Superoxide dismutase, abbreviated as SOD, is a metalloenzyme that specifically scavenges oxygen free radicals, and can continuously replenish exogenous SOD in the body through the skin action pathway. Can effectively prevent and delay the occurrence of skin aging process. Clinical verification and long-term application have shown that SOD not only has significant effects such as anti-wrinkle, freckle and pigmentation, but also anti-inflammatory, sunscr...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K38/44A61K47/48A61K47/26A61K47/10A61P17/18A61K47/60
CPCA61K9/1271A61K9/0014A61K38/446A61K47/10A61K47/26
Inventor 刘娟
Owner AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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