A method for cultivating insect-resistant transgenic corn

Abstract

The invention discloses a method for cultivating insect-resistant transgenic maize. The method for cultivating insect-resistant transgenic maize comprises the step that an insect-resistant gene is guided into target maize to obtain the transgenic maize expressing the insect-resistant gene. Compared with the target maize, the insect resistance of the transgenic maize is improved. The insect-resistant gene is DNA molecules shown in a or b or c, wherein the nucleotide sequence a is shown in the sequence 3 in a sequence table, the nucleotide sequence b is shown in 1st-2007th bits of the sequence 3 in the sequence table, and the nucleotide sequence c and the sequence 3 or the 1st-2007th bits of the sequence 3 in the sequence table at least have the 98% identity, and protein is shown in the coded sequence 9. Compared with the transgenic maize containing the transferred Cry1Ah gene before optimization, the transgenic maize containing the artificially-synthetized insect-resistant gene has the advantages that the expression quality of Cry1Ah protein is obviously improved, and meanwhile the insect resistance is obviously improved.

Description

technical field The invention relates to a method for cultivating insect-resistant transgenic corn. Background technique Most of the exogenous genes used in plant transgenic breeding, such as Bt and EPSPS, are from prokaryotes. Due to the characteristics of prokaryote genes, such as 1) AT content is higher, exceeding 60%, causing the mRNA of gene expression to be in the plant. It is easily degraded in the body; 2) There are intron cut points and transcription terminator sequences similar to eukaryotic genes, resulting in incomplete transcription, abnormal splicing of mRNA, etc.; 3) There are large differences between codons and plant codons, resulting in The protein translation efficiency is reduced; 4) its structure is significantly different from that of plants and other eukaryotic organisms, such as the 5'-UTR sequence and the polyA tailing sequence at the 3' end that do not contain eukaryotic genes, resulting in lower gene expression levels in plants. Low. For example,...

AI Technical Summary

Problems solved by technology

Since the endogenous insect resistance of maize is controlled by multiple genes, and the target pest genes at the heart leaf stage and the ear stage are independently inherited, it is not only long-term to breed borer-resistant hybrids with conventional breeding methods, but it is also difficult to obtain concurrently resistant Parent of two generations of corn borer

At the same time, the maize borer resistance gene is likely to be negatively correlated with high-yield traits, and no significant progress has been made in the breeding of borer resistance in various countries in the past 20 years

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