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A kind of tissue culture and rapid propagation method of mountain red leaves and yellow eight feet

A technology of tissue culture rapid propagation and red leaves, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low germination rate and survival rate, low reproduction coefficient, long reproduction cycle, etc., and achieve the goal of cultivating time Short, simple cultivation process, simple formula effect

Active Publication Date: 2017-10-20
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Shanhongye Huangbazhang is mainly propagated by sowing and grafting, but the germination rate and survival rate are not very high, the reproduction coefficient is low, the reproduction cycle is long, the site requirements are large, and the labor cost is high, which is far from meeting the domestic demand for seedlings.

Method used

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  • A kind of tissue culture and rapid propagation method of mountain red leaves and yellow eight feet
  • A kind of tissue culture and rapid propagation method of mountain red leaves and yellow eight feet
  • A kind of tissue culture and rapid propagation method of mountain red leaves and yellow eight feet

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Experimental program
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Effect test

Embodiment 1

[0031] A kind of tissue culture rapid propagation method of Huangbazhang described in the present embodiment comprises the following steps:

[0032] (1) Select the young and tender branches of Shanhongye Huang Bazhang, remove the leaves, cut into 2-3cm long bud stem segments, and treat them with 75% alcohol for 8-15s on the sterile operating table, and then use 0.1% alcohol Mercury chloride treatment for 8-13 minutes, rinsed with sterile water for 4-6 times, and then dried to obtain explants;

[0033] (2) Initiate culture: inoculate the explants obtained in step (1) into an initiating medium with a pH value of 6.3-6.7 and cultivate for 3-5 weeks to produce axillary buds. The composition of the initiating medium is: MS basal medium , supplemented with 0.02mg / L IAA, 0.2mg / LKT, 30g / L sucrose, 5g / L agar;

[0034] (3) Proliferation culture: cut out the axillary buds obtained in step (2), inoculate them into the proliferation medium and cultivate them for 6-8 weeks, and cluster bud...

Embodiment 2

[0039] The tissue culture rapid propagation method of a kind of mountain red leaf Huang Bazhang described in the present embodiment, the difference with embodiment 1 is:

[0040]In step (2), the composition of starting medium is: MS minimal medium supplemented with 0.01mg / L IAA, 0.1mg / LKT, 10g / L sucrose, 1g / L agar;

[0041] In step (3), the composition of the proliferation medium is: B5 basic medium, supplemented with 0.05mg / L NAA, 0.05mg / LKT, 0.1mg / L 6-BA, 100mg / L CH;

[0042] In step (4), the composition of the rooting medium is: 1 / 2B5 basic medium, supplemented with 0.5mg / L IBA.

[0043] In the present embodiment, during the start-up culture process, the time for the first axillary bud to form was 31 days, and when the start-up culture ended, the axillary bud elongated by 1-2 cm; during the proliferation culture process, the time for the first clustered bud to form was 68 days, At the end of the proliferation culture, the height of the clustered buds is 1-3cm, and the prol...

Embodiment 3

[0045] The tissue culture rapid propagation method of a kind of mountain red leaf Huang Bazhang described in the present embodiment, the difference with embodiment 1 is:

[0046] In step (2), the composition of starting medium is: MS minimal medium supplemented with 0.08mg / L IAA, 0.6mg / LKT, 50g / L sucrose, 8g / L agar;

[0047] In step (3), the composition of the proliferation medium is: B5 basic medium, supplemented with 0.25mg / L NAA, 0.25mg / LKT, 1.0mg / L 6-BA, 800mg / L CH;

[0048] In step (4), the composition of the rooting medium is: 1 / 2B5 basic medium, supplemented with 0.1mg / L IBA.

[0049] In the present embodiment, during the start-up culture process, the time for the first axillary bud to form is 40 days, and when the start-up culture ends, the axillary bud elongates by 1-2 cm; during the proliferation culture process, the time for the first clustered bud to form is 64 days, At the end of the proliferation culture, the height of the clustered buds is 1-3cm, and the prolif...

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Abstract

The invention discloses a tissue culture fast propagation method of acer palmatum 'Ki-hachijo'. The method comprises the following steps that 1, explants are selected and sterilized, wherein the stems with buds of acer palmatum 'Ki-hachijo' are cut and sterilized through ethyl alcohol and mercury bichloride to obtain the explants; 2, initiated culture is carried out, wherein the explants obtained in the step 1 are inoculated in an initiated culture medium to be cultured for 3-5 weeks, and axillary buds are generated; 3, enrichment culture is carried out, wherein the axillary buds obtained in the step 2 are cut and inoculated in an enrichment culture medium to be cultured for 6-8 weeks, and cluster buds are generated; 4, rooting culture is carried out, wherein the cluster buds obtained in the step 3 are separated into single buds, and the single buds are inoculated in a rooting culture medium to be cultured for 3-4 weeks to obtain acer palmatum 'Ki-hachijo' aseptic seedlings. The adopted culture media are simple in formula, the tissue culture process is simple and convenient, the culture time is short, the propagation frequency is high, the seedlings are in order, unified transplanting and planting management in the later period is facilitated, the labor cost is reduced, it can be ensured that growing seedlings are consistent in shape, operation is easy, and industrial production can be carried out.

Description

technical field [0001] The invention relates to the technical field of biological group cultivation seedlings, in particular to a method for tissue culture and rapid propagation of mountain red leaves and yellow bazhang. Background technique [0002] Its Latin name is Acer palmatum'Ki-hachijo'. Its plants are strong and its leaves are large and bright dark green. The color of autumn leaves is very special, eye-catching golden yellow with a little rose red. Turns into a mixture of light orange and red, and can be planted alone or in groups, and its plants are strong and vigorous. Although this species is not widely planted at present, it will become a very popular landscape tree species with great ornamental value and economic value. At present, Shanhongye Huangbazhang is mainly propagated by sowing and grafting, but the germination rate and survival rate are not very high, the reproduction coefficient is low, the reproduction cycle is long, the site requirements are large, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 沈香兰王小辉马良良马建华杨小霞
Owner SICHUAN COLORLINK CO LTD