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Method for determining alkaloids in tea leaves by using GC-MS (Gas Chromatography-Mass Spectrometer) method

A GC-MS, alkaloid technology, applied in the determination of nicotine, anatine, neonicotine and cotinine in tea, mesmin, nornicotine field, can solve the problem of few determination methods and inability to directly Using the problems of tea alkaloid content detection and cumbersome methods to achieve the effect of optimizing instrument detection conditions, short detection time and good repeatability

Inactive Publication Date: 2016-04-06
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a common food in people's daily life, tea is very meaningful for the determination of alkaloids contained in it, but there are few methods for the determination that have been published. There have been reports in the literature that nicotine can be detected in tea, but the method is relatively cumbersome
[0004] "YC / T383-2010 Determination of Nicotine, Nornicotine, Anonicotine, Mesmin and Pseudine in Tobacco and Tobacco Products by Gas Chromatography-Mass Spectrometry" developed a detection method for tobacco products, but nicotine in tobacco The content of 3% in tea is much higher than that in tea (20μg / kg), so this method cannot be directly used for the detection of alkaloid content in tea

Method used

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  • Method for determining alkaloids in tea leaves by using GC-MS (Gas Chromatography-Mass Spectrometer) method
  • Method for determining alkaloids in tea leaves by using GC-MS (Gas Chromatography-Mass Spectrometer) method
  • Method for determining alkaloids in tea leaves by using GC-MS (Gas Chromatography-Mass Spectrometer) method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0050] 1. Reagents and instruments:

[0051] Standard products: 2-methylquinoline, nicotine, nornicotine, mesmin, pseudobasine, anatine and cotinine;

[0052] Reagents: triethylamine, chloroform, 5% sodium hydroxide solution, methyl tert-butyl ether;

[0053] Instruments: gas chromatography mass spectrometry (Agilent); fused silica capillary column (30mx0.25mmx0.25μm); vortex oscillator, (Advancedmulti company) desktop high-speed refrigerated centrifuge (SIGMA company); AE163 electronic balance, sense: 0.0001g, (Mettler, Switzerland). Organic phase syringe filter, 13mm×0.22μm (Shanghai Anpu Scientific Instrument Co., Ltd.).

[0054] 2. Sample handling:

[0055] Weigh 2g of tea powder sample into a 50mL centrifuge tube, then add 1.0~6.0mL of 10% sodium hydroxide solution, and let stand for 10min. Take 10mL of 0.01% triethylamine in methyl tert-butyl ether solution (extraction solution) into a centrifuge tube, and add 200μL of internal standard solution (the concentration ...

example 2

[0067] The specific steps are as described in Example 1, another sample B is selected, and the measured content is shown in Table 5.

[0068] The detection result in the tealeaves of table 5

[0069] compound

example 3

[0071] The specific steps are as described in Example 1. Another sample C was selected, and the measured nicotine content is shown in Table 6.

[0072] The detection result in table 6 tealeaves

[0073] compound

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Abstract

The invention belongs to the technical field of physiochemical detection of tea leaves and in particular relates to a method for determining alkaloids in tea leaves by using a GC-MS (Gas Chromatography-Mass Spectrometer) method. The method comprises the following step of determining nicotine and minor alkaloids (including nicotine, nornicotine, myosmine, neonicotine, neonicotine and cotinine) in tea leaf powder. The method is a method for extracting nicotine substances from the tea leaves by using a centrifugal tube filled with 0.01% triethylamine / tert-butyl methyl ether solution and analyzing the nicotine substances in the tea leaves by using a gas chromatography-mass spectrometer (GC-MS) method. When the method provided by the invention is used for detecting the content of the nicotine in the tea leaves, the method is rapid and effective, the pre-treatment is simple, an average relative standard deviation is smaller than 10 percent, and the average recycling rate of each index is 86.2 percent to 92.1 percent. The method has the advantages of rapidness and accuracy, high sensitivity and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing of tea, and specifically relates to a method for measuring alkaloids in tea using GC-MS, and relates to Determination techniques for base and cotinine. Background technique [0002] Tobacco alkaloids are an extremely important class of chemical components in tobacco. Nicotine, nornicotine, anatabine, and anatabine are the main alkaloids, and other alkaloids except nicotine are usually called "secondary alkaloids". Nicotiana belongs to the family Solanaceae Nicotiana, which can be divided into 3 subgenera, namely common tobacco, Nicotiana nicotiana and Pidonga, a total of 66 species in 14 groups. The alkaloid content varies greatly. Among them, 50-60% of the species use nicotine as the main alkaloid, and nicotine accounts for more than 80% of the total alkaloids, including common tobacco and Nicotiana japonica which are valuable for cultivation and utilization. 30-40% of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 张洪非朱风鹏姜兴益李雪罗彦波李翔宇庞永强陈欢韩书磊刘彤付亚宁刘洋
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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