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Castanopsis hystrix high efficiency isolated culture plant regeneration method

An in vitro culture, red cone technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as tissue culture technology that has not yet been reported, and achieve high rooting rate, developed root system, and transplant survival rate. high effect

Active Publication Date: 2016-04-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There has not been any report on the tissue culture technique using the seeds of the red cone tree as explants

Method used

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  • Castanopsis hystrix high efficiency isolated culture plant regeneration method
  • Castanopsis hystrix high efficiency isolated culture plant regeneration method
  • Castanopsis hystrix high efficiency isolated culture plant regeneration method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment one adopts the following steps to realize the in vitro culture plant regeneration of red cone:

[0040] (1) Induction of adventitious buds from seeds

[0041] Preparation and treatment of explants: Peel off the shell of the selected excellent tree seeds in advance with branch shears, and peel off the inner and outer seed coats with a scalpel on the ultra-clean workbench, paying attention to ensure the integrity of the embryo.

[0042] Surface disinfection of explants: first scrub the seeds with sterile water on the ultra-clean workbench, then use alcohol with a volume percentage concentration of 75%, soak for 20s, then rinse with sterile water once, and then add mass volume percentage Concentration of 0.05% mercuric chloride solution, soak for 2 minutes, shake constantly during this time, drain the mercuric liter solution and rinse with sterile water 6 times. Blot the water droplets on the surface of the sterile material with sterile filter paper for later u...

Embodiment 2

[0050] Embodiment two adopts the following steps to realize the in vitro culture plant regeneration of red cone:

[0051] (1) Induction of adventitious buds from seeds

[0052] Preparation and treatment of explants: Peel off the shell of the selected excellent tree seeds in advance with branch shears, and peel off the inner and outer seed coats with a scalpel on the ultra-clean workbench, paying attention to ensure the integrity of the embryo.

[0053] Disinfection of the surface of the explants: first scrub the seeds with sterile water on the ultra-clean workbench, then use alcohol with a volume percentage concentration of 72%, soak for 30s, then rinse with sterile water twice, and then add the mass volume percentage Concentration of 0.1% mercuric chloride solution, soak for 1min, shake continuously during this period, drain the mercuric chloride solution and rinse with sterile water 5 times. Blot the water droplets on the surface of the sterile material with sterile filter ...

Embodiment 3

[0061] Embodiment three adopts the following steps to realize the in vitro culture plant regeneration of red cone:

[0062](1) Induction of adventitious buds from seeds

[0063] Preparation and treatment of explants: Peel off the shell of the selected excellent tree seeds in advance with branch shears, and peel off the inner and outer seed coats with a scalpel on the ultra-clean workbench, paying attention to ensure the integrity of the embryo.

[0064] Disinfection of the explant surface: scrub the seeds with sterile water on the ultra-clean workbench, then use alcohol with a volume percentage concentration of 70%, soak for 30s, and then rinse with sterile water once, and then add the mass volume percentage Concentration of 0.1% mercuric chloride solution, soak for 2 minutes, shake continuously during this period, pour out the mercuric liter solution and rinse with sterile water for 6 times. Blot the water droplets on the surface of the sterile material with sterile filter p...

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Abstract

The invention belongs to the technical field of plant tissue culture and discloses a Castanopsis hystrix high efficiency isolated culture plant regeneration method. Through explant treatment, basic medium design and optimization of different hormone types and a concentration level ratio, a Castanopsis hystrix select tree seed is used as an explant and is subjected to adventitious bud induction, bud propagation culture and rooting culture to form a complete plant. The invention discloses a Castanopsis hystrix tissue culture rooting seedling transplanting matrix and a management technology of a transplanted seedling. The method fills the blank of tissue culture rapid propagation of a Castanopsis hystrix select tree seed as an explant, provides a stable and efficient Castanopsis hystrix isolated culture plant regeneration method, can be industrialized, has a low production cost and can culture strong, tidy and uniform Castanopsis hystrix seedlings. Castanopsis hystrix belongs to precious tree species. At present, an afforestation area is large and a market high-quality nursery stock is deficient. The Castanopsis hystrix high efficiency isolated culture plant regeneration method can provide technical support and fine breeding guarantee for fast promotion of high-quality Castanopsis hystrix germplasm and improvement of Castanopsis hystrix artificial forest economic benefits and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a plant regeneration method for high-efficiency in vitro culture of red cones. Background technique [0002] Castanopsishystrix A.DC belongs to the genus Castanopsis of Fagaceae. The adult tree height can reach 30m, and the diameter at breast height is more than 1m. It is an important local broad-leaved precious timber and high-efficiency multi-purpose ecological public welfare forest tree species. Red cones are naturally distributed in east longitude 95°20′~118°00′ and north latitude 18°30′~25°00′. The main production areas are concentrated in Guangdong, Guangxi, and southern Fujian, and its surrounding areas are distributed in Hainan, southern Yunnan, and southeastern Guizhou. And Hunan, Jiangxi and other provinces in the south. [0003] Red cone has excellent characteristics such as fast growth, excellent material, wide adaptability and high benefit....

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008Y02A40/22Y02P60/40
Inventor 奚如春李蕊萍邓小梅焦金凤赵梦秋郑珂媛
Owner SOUTH CHINA AGRI UNIV
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